Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1367-1370.doi: 10.3969/j.issn.1673-8225.2012.08.009

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Component analysis of a natural acellular sciatic nerve scaffold 

Cao Rong-long, Li Chang-yu, Zhang Xiang-tong   

  1. Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Received:2011-07-21 Revised:2011-11-26 Online:2012-02-19 Published:2012-02-19
  • Contact: Zhang Xiang-tong, Professor, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China zgxgtg@sina.com.cn
  • About author:Cao Rong-long★, Studying for master’s degree, Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China caoronglong241@ 163.com

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Abstract:

BACKGROUND: Currently, there are so many acellular methods used in the peripheral nerve, and two common methods are chemical extraction and frozen-thawing method. However, chemical extraction has the disadvantage of time-consuming and frozen-thawing method cannot achieve complete cell-free. Freeze-thaw+chemical extraction+mechanical vibration were chosen as optimization method for acellular processing of sciatic nerve in this study based on a large amount of literatures and repeated practice.
OBJECTIVE: To analyze the morphology and composition of the natural acellular sciatic nerve scaffold prepared by optimization method.
METHODS: Bilateral sciatic nerves from Wistar rats were randomly divided into two groups. The control group did not receive special treatment. The experimental group was treated with freeze-thaw+chemical extraction+mechanical vibration to prepare acellular scaffold.
RESULTS AND CONCLUSION: Schwann cells, myelin sheaths and axons were removed completely from the optimized acellular sciatic nerve scaffold. And the scaffold showed a three-dimensional tubular structure. Extracellular matrixes of type Ⅰ collagen, type Ⅲ collagen and laminin were retained.

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