Abstract:

BACKGROUND: Allogeneic transplantation of neural stem cells in cell replacement therapy is the hot spots for the treatment of Parkinson’s disease (PD). But the prior studies mainly focus on the morphological information of transplanted cells and animal behavior change, and the mechanism is unclear.
OBJECTIVE: To analyze the mechanism of neuroepithelial stem cells transplantation for the treatment of PD through the research of the relationship between the rotation behavior change and the content of dopamine of PD rats after neuroepithelial stem cells transplantation. 
METHODS: The PD rat models were established and divided into two groups. The neuroepithelial stem cells derived from green fluorescent protein in experimental group were transplanted into striatum of PD rat model after clone, and then rat in the control group was injected with normal saline. The survival and differentiation of transplant neuroepithelial stem cells were detected and the relationship between the change of rotation behavior and content of dopamine were analyzed.
RESULTS AND CONCLUSION: The rotation behavior of rat in the experimental group was improved significantly after transplantation. Some grafts were found survived well and some TH-positive cells could be detected in the graft region. The content of dopamine in experimental group was significantly higher than that in control group. The recovery of PD rat rotational behavior was positively related to dopamine content change after the transplantation of neuroepithelial stem cells carrying green fluorescent protein, the differentiation of functional TH-positive cells may be the basic mechanism of neuroepithelial stem cells transplantation for the treatment of PD.