Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1041-1045.doi: 10.3969/j.issn.1673-8225.2012.06.021

Previous Articles     Next Articles

Roles of fluorescence in situ hybridization in allogeneic hematopoietic stem cell transplantation

Mo Yao-xi1, Zhou Rui-lian2, Mo Wei-ying1, Lan Mei2, Lin Jin-ying2   

  1. 1Reproductive Medicine and Genetic Center, 2Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-13 Revised:2011-07-04 Online:2012-02-05 Published:2012-02-05
  • Contact: Lin Jin-ying, Master, Chief physician, Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China jinyinglin@sina.com
  • About author:Mo Yao-xi★, Master, Research assistant, Reproductive Medicine and Genetic Center, the People’s Hospital of Guangxi Zhuang autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China moson1_ok@yahoo.com.cn Zhou Rui-lian, Master, Attending physician, Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China qianzilian117@163.com
  • Supported by:

     the Health Bureau of Guangxi Zhuang Autonomous Region, No. Z2008057*, Z2009133*

PDF

385

Abstract:

BACKGROUND: The chimeric rate is very important in the allogeneic hematopoietic stem cell transplantation. Its decline always means the host anti-graft or leukemia relapse. Many techniques, including bone marrow cytology, conventional cytogenetic and flow cytometry, are not sensitive and specific enough in monitoring the chimerism.
OBJECTIVE: To verify the sensitivity and specificity of fluorescence in situ hybridization (FISH) in the detection of allogeneic chimerism after allogeneic hematopoietic stem cell transplantation.
METHODS: The chimerism of the sex mismatched Allo-HSCT was detected by FISH, utilizing X, Y chromosomes and bcr/abl fusion gene, in comparison with bone marrow cytology.
RESULTS AND CONCLUSION: Through monitoring the chimerism by FISH in 3 cases, which were sex mismatched Allo-HSCT, it is confirmed that FISH is more sensitive and specific than bone marrow cytology, and has the unique advantage in detection of chimerisms.

CLC Number: