Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1024-1027.doi: 10.3969/j.issn.1673-8225.2012.06.017

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Heme oxygenase-1 can improve the survival rate of canine cord blood-derived mesenchymal stem cells transplanted into infarcted hearts

Chen De-hai1, Wang Ping1, Zhang Shen1, Wu Lian-he1, Zhang Pei-xi1, Ma Nan2   

  1. 1Department of Cardiothoracic Surgery, First People’s Hospital of Jining, Jining  272111, Shandong Province, China; 2Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai  200092, China
  • Received:2011-06-13 Revised:2011-07-14 Online:2012-02-05 Published:2012-02-05
  • About author:Chen De-hai☆, Doctor, Associate chief physician, Department of Cardiothoracic Surgery, First People’s Hospital of Jining, Jining 272111, Shandong Province, China cdh255@126.com
  • Supported by:

    the Project of Jining Science and Technology Committee, No. 2009-56-2-9*

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Abstract:

BACKGROUND: The heme oxygenase-1 has a strong endogenous antioxidant role, and can reduce inflammation and play a protecting role for cells. 
OBJECTIVE: To study the effect of heme oxygenase-1 on the survival rate of canine cord-derived mesenchymal stem cells transplanted in infarcted hearts.
METHODS: Cord blood was obtained, and mononuclear cells were isolated by density gradient centrifugation and amplified by adherent culture. Mesenchymal stem cells of the fourth generation were treated by hemin for 24 hours, then the expression of heme oxygenase-1 enzyme was detected by immunohistochemistry. The fourth generation cells labeled by the LacZ reporter gene were transplanted into infracted area of hearts.
RESULTS AND CONCLUSION: The heme oxygenase-1 expression of canine cord-derived mesenchymal stem cells following pretreated with hemin was significantly increased for more than 14 days. The cells transplanted into the infarcted area could survive for a long time. Image analysis showed that the 4, 8 weeks after transplantation, cell viability of the experimental group was significantly higher than control group. Preliminary results suggest that over-expression of heme oxygenase-1 can significantly increase the survival of cord-derived mesenchymal stem cells after transplanted into myocardial infarction hearts.

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