Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1011-1014.doi: 10.3969/j.issn.1673-8225.2012.06.014

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Comparison and screening of prostate cancer stem cells isolation methods********★

Li Kui-qing, Xu Ke-wei, Zhou Bang-fen, Fan Xin-lan, Dong Wen, Zhang Cai-xia, Bi Liang-kuan   

  1. Department of Urinary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
  • Received:2011-07-14 Revised:2011-08-10 Online:2012-02-05 Published:2012-02-05
  • Contact: Xu Ke-wei, Master, Associate professor, Department of Urinary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China xkw2005@163.com
  • About author:Li Kui-qing★, Studying for master’s degree, Department of Urinary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China kuiqing04@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 81001138*; the National Natural Science Youth Foundation of China, No. 30901488*; the New Teacher Foundation of Ministry of Education, No. 200805581124*; the Natural Science Foundation of Guangdong Province, No. 06021283*, 1015100 8901000024*; the Science and Technology Development Program of Guangdong Province, No. 2008B030301078*; the Natural Science Foundation for Doctors of Guangdong Province, No. 94510 08901003001*; the Medical Science and Technology Research Foundation of Guangdong Province, No. A2008189*

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Abstract:

BACKGROUND: Prostate cancer stem cell is an important reason for the invasion and recurrence of prostatic carcinoma. However, separation efficiency of prostate cancer stem cells is very low.
OBJECTIVE: To explore the efficient method for isolating and identifying the prostate cancer stem cells from human prostatic carcinoma cell lines PC-3 and LNCap.
METHODS: Prostate cancer cell lines PC-3 and LNCap were cultured in serum free medium (SFM) and serum supplemented medium (SSM), respectively. The percentage of prostate cancer stem cells from different medium was detected by flow cytometry through markers CD133 and CD44, and the properties of prostate cancer stem cells were preliminarily identified using inducing differentiation experiments.
RESULTS AND CONCLUSION: PC-3 and LNCap formed sphere cells in SFM, which can be induced into adherent cells after culture in SSM. Higher percentage of CD44+/CD133+cells was obtained from LNCap cells (1.71%; 0.73%) than PC-3 cells (0.59%; 0.32%) in both SFM and SSM. The number of CD44+/CD133+ LNCap cells was more than PC-3 using both methods (P < 0.05), but the efficiency of SFM and SSM had no statistical significance (P > 0.05). However, the culture cycle was longer and number of obtained cells was less by SFM culture, directly influencing functional determination of prostate cancer stem cells. Compared with suspension culture method with SFM, SSM is more convenient and effective in isolating prostate cancer stem cells from LNCap cells.

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