Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 951-957.doi: 10.3969/j.issn.1673-8225.2012.06.001

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Security and magnetic resonance imaging features of superparamagnetic iron oxide nanoparticles labelling rabbit bone marrow mesenchymal stem cells

Meng Zeng-dong1,2, Qiu Wei1,2, Hu Biao1,2, Liu Wei1, 2, Dai Min-fang1, 2, Lei Yun-kun1,2   

  1. 1Department of Orthopedics, Affiliated Kunhua Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China; 2Department of Orthopedics, Yunnan First People’s Hospital, Yunnan  650032, Yunnan Province, China
  • Received:2011-07-22 Revised:2011-11-01 Online:2012-02-05 Published:2012-02-05
  • Contact: Qiu Wei, Master, Department of Orthopedics, Affiliated Kunhua Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China; Department of Orthopedics, Yunnan First People’s Hospital, Yunnan 650032, Yunnan Province, China tlger111@163.com
  • About author:Meng Zeng-dong☆, Doctor, Associate chief physician, Department of Orthopedics, Affiliated Kunhua Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China; Department of Orthopedics, Yunnan First People’s Hospital, Yunnan 650032, Yunnan Province, China menggu7119@vip.sina.com
  • Supported by:

    the Application Basic Research Foundation of Yunnan Province, No.2010CD195*

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Abstract:

BACKGROUND: The traditional methods to label stem cells often need histopathology examination. It can not observe the whole experiment process dynamically and is not suitable for clinical research. 
OBJECTIVE: To observe the biological characteristics of rabbit bone marrow mesenchymal stem cells (BMSCs) labeled by superparamagnetic iron oxide (SPIO) nanoparticles in vitro, and to observe the magnetic resonance imaging (MRI) features of labeled BMSCs in vitro.
METHODS: Rabbit BMSCs were cultured and expanded by using red blood cells lysis and adherent in vitro. Rabbit BMSCs were labeled with SPIO of different concentrations (100, 50, 25, 12.5 mg/L) combined with polylysine (PLL) (0.75 mg/L). The MRI imaging features of labeled rabbit BMSCs were observed by MRI scanning.
RESULTS AND CONCLUSION: The rabbit BMSCs labeled with 25 mg/L SPIO combined with 0.75 mg/L PLL were more efficient and safe. This level of concentration could not influence on cells activity and could not influence the osteogenic and adipogenic differentiation capacity of labeled rabbit BMSCs obviously. MRI scanning on T2 * WI sequences can show the SPIO nanoparticles labeled rabbit BMSCs significantly and effectively.

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