Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 471-474.doi: 10.3969/j.issn.1673-8225.2012.03.020

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Effects of poly(lactic-co-glycolic acid)/type-Ⅰcollagen/chitosan composite membrane in rabbit models of spinal cord injury 

Bai Wan-shan, Wang Xin-wei, Yuan Wen, Wang Zhan-chao, Liang Lei, Wang Hui-xue   

  1. Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai  200003, China
  • Received:2011-07-14 Revised:2011-09-15 Online:2012-01-15 Published:2012-01-15
  • Contact: Wang Xin-wei, Doctor, Associate chief physician, Associate professor, Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai 200003, China
  • About author:Bai Wan-shan☆, Doctor, Attending physician, Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai 200003, China baiwanshan_01@ 163.com
  • Supported by:

     

    the Science and Technology Research Plan of Shanghai Science and Technology Committee, No. 08411963800*

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Abstract:

BACKGROUND: Autologous tissue, allograft tissue, and dural substitute materials originated from animals are all difficult to reduce the morbidity and mortality of spinal cord injury.
OBJECTIVE: To study the effects of poly(lactic-co-glycolic acid) (PLGA)/type-Ⅰcollagen/chitosan composite membrane in rabbit models with spinal cord injury.
METHODS: Seventy rabbits were randomly divided randomly into sham-operation group (n=10, simple laminectomy without spinal cord injury), model group (n=20, spinal cord injury without treatment), chitosan group (n=20, spinal cord injury treated with artificial dura mater of chitosan), and composite group (n=20, spinal cord injury treated with PLGA/type-Ⅰcollagen/chitosan composite membrane).
RESULTS AND CONCLUSION: Twenty-four hours after spinal cord injury, the motor behavior scores was higher in the chitosan group and composite group than model group (P < 0.01), and the scores in the composite group were also higher than those in the chitosan group (P < 0.05). The latency of somatosensory evoked potential was longer in the model, chitosan, and composite groups than the sham-operation group after spinal cord injury. The latencies were increased at 6 hours after spinal cord injury and reached the peak at 24 hours, and then began to decrease. Two days after spinal cord injury, the differences in the latencies were insignificant among the model, chitosan and composite groups. The apoptotic rate of the sham-operation group was lower than that of other groups (P < 0.05). At 6 and 24 hours after spinal cord injury, the apoptotic rates of the chitosan and composite groups were lower than that of the model group (P < 0.05). Early surgical intervention with PLGA/type-Ⅰcollagen/chitosan composite membrane can acquire best neurological functional recovery in rabbit models of spinal cord injury.
 

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