Abstract:

BACKGROUND: Type I collagen is not only a major component of the cornea, but also can repair the injured cornea and play the role of nutrition, with a good drug delivery capability.
OBJECTIVE: To understand the effect of collagen eye drop on the repairing of injured rabbit’s corneal and to evaluate the biological safety.   
METHODS: Corneal Ring Drill was used for rabbit cornea injury model preparation. Anti-infection treatment was performed after surgery. Right eyes treated with collagen eye drop were used as experimental group, and left eyes treated with normal saline served as controls. Secretion and edema degrees, 2% fluorescent element corneal dyeing ratings, damage healing rates were used for evaluation of the effect of collagen eye drop on cornea injury.
RESULTS AND CONCLUSION: The conjunctival secretions in the experimental group were greatly lower than that in the control group. Compared with the control group, the edema and congestions of rabbits after surgery were lighter in the experimental group. The healing rates of the cornea lesion at 11 and 13 days after surgery in the experimental group were significantly higher than those in the control group (P < 0.05). In vitro cytotoxicity test showed not more than 2 levels of reaction. And there was no allergic reaction, and the results showed that the collagen eye drops had no biological hazards and could accelerate the wound healing of the cornea.