Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 455-458.doi: 10.3969/j.issn.1673-8225.2012.03.016

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Cellular biocompatibility of silk fibroin/chitosan composite scaffolds 

She Rong-feng, Deng Jiang, Huang Wen-liang, Dong Zhi-jun, Liu Bin   

  1. Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2011-07-20 Revised:2011-08-24 Online:2012-01-15 Published:2012-01-15
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China DJ30666@126.com
  • About author:She Rong-feng★, Studying for master’s degree, Physician, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Guizhou Province, No. [2009]2172*; a grant from the Science and Technology Bureau of Zuiyi City, No. [2010]15*

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Abstract:

BACKGROUND: Many studies show that silk fibroin (SF) and chitosan (CS) are natural polymer materials that have good biocompatibility. 
OBJECTIVE: To discuss the biocompatibility of induced rabbit bone marrow mesenchymal stem cells (BMSCs) with SF/CS composite scaffolds.
METHODS: Rabbit BMSCs were isolated, cultured and induced, and then cocultured with three-dimensional SF/CS scaffolds   in vitro. Cell toxicity of scaffolds, cell viability, cell adhesion rate were determined to evaluate cellular biocompatibility of the materials as well as scanning electron microscope (SEM) observation.
RESULTS AND CONCLUSION: After being induced, the rabbit BMSCs adhered and grew well on the scaffolds to keep normal division and proliferation. As time went, the cell adhesion rate increased and the scaffolds group had a stronger adhesion rate than the control group, and the difference had statistic meaning (P < 0.05). The SEM observation found that the cells grew well after 48 hours, which were closely adhered to scaffolds as well as divided and proliferated actively. The above findings indicate that the three-dimensional SF/CS scaffolds have a better cellular biocompatibility.

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