Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 434-437.doi: 10.3969/j.issn.1673-8225.2012.03.012

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Enhanced osteogenesis of implantation of heterogeneous demineralized bone matrix combined with bone marrow mesenchymal stem cells into the tympanic bullas of guinea pigs  

Duan Li-fu1, Wan Hai-tao2, Li Hua-jing1, Ma Yan3, Liu Li-zhong1   

  1. 1Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 2Imaging Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 3Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-07-29 Revised:2011-10-20 Online:2012-01-15 Published:2012-01-15
  • Contact: Liu Li-zhong, Doctor, Professor, Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China lulizhongno.1@ 163.com
  • About author:Duan Li-fu★, Studying for master’s degree, Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China 1458668171@ qq.com
  • Supported by:

    Graduate Innovation Foundation of Xinjiang Medical University, No. MCR010-7*

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Abstract:

BACKGROUND: Currently, there are studies on the related applications of demineralized bone matrix and bone marrow mesenchymal stem cells (BMSCs) in orthopedic surgery and craniofacial reconstruction. However, the application in mastoid obliteration is still unclear.
OBJECTIVE: To explore the osteogenic effectiveness of heterogeneous demineralized bone matrix combined with BMSCs implanted into the mastoid cavity.
METHODS: Inbred female guinea pig BMSCs were isolated with the whole bone marrow adherent culture method, and the demineralized bone matrix was prepared with cancellous bone of bovine femoral head. Twenty guinea pigs were equally divided into two groups: the experimental group was implanted by demineralized bone matrix combined with BMSCs into the tympanic bulla, and the control group was treated with implantation of autologous lliac into the tympanic bulla.
RESULTS AND CONCLUSION: The new bone quantity in the two groups had no significant difference at 1 month after implantation; 2 months later, the new bone quantity in the experimental group was significantly higher than that of the control group (P < 0.05). It is indicated that the implantation of heterogeneous demineralized bone matrix combined with BMSCs into the mastoid cavity can effectively promote the osteogenesis after mastoid obliteration.

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