Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 396-400.doi: 10.3969/j.issn.1673-8225.2012.03.003

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Preparation of poly lactic acid-glycolic acid nanoparticles  

Wang Xi1, Xue Jing2, Huang Yue-shan2   

  1. 1Guangdong Food and Drug Vocational College, Guangzhou  510520, Guangdong Province, China; 2College of Bioscience and Bioengineering, South China University of Technology, Guangzhou  510006, Guangdong Province, China
  • Received:2011-07-14 Revised:2011-11-16 Online:2012-01-15 Published:2012-01-15
  • Contact: Huang Yue-shan, Associate professor, College of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China bmbyshan@ scut.edu.cn
  • About author:王希，女，1965年生，山东省威海市人，汉族，1988年中国药科大学毕业，副教授，主要从事医药和化工的研究。 zheng5@ sina.com
  • Supported by:

    General Program of Natural Science Foundation of Guangdong Province, No. 9151052005000006* 
       

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Abstract:

BACKGROUND: Poly lactic acid-glycolic acid (PLGA) is a type of biodegradable material with good biocompatibility. Optimum preparation conditions of PLGA are conductive to the follow-up studies of drug delivery and their establishment of industrial production conditions.
OBJECTIVE: To explore the effects of preparation conditions of nanoparticles based on the wrapping material of PLGA on their particle size and surface morphology, and determine the optimum preparation and process.
METHODS: PLGA nanoparticles were prepared using emulsion-solvent evaporation technique. The particle size of nanoparticles was measured. The effects of emulsifier type, emulsifier concentration, oil phase type, sonic time, volatile time, the volume ratio of water to oil, and polymer concentration on the particle size were analyzed to establish the optimum preparation conditions of PLGA.
RESULTS AND CONCLUSION: The optimization parameters at the room temperature, with a certain stirring speed and drop acceleration, selection of the commonly used non-toxic emulsifier, the concentration was from 0.3% to 1%, acetone as organic phase, extraction time was from 8 to 15 minutes, volatile time was from 6 to 10 hours, ratio of water and oil > 25:1, and the polymer concentration < 60 g/L. The preparation process is simple, stable and optimized. The PLGA nanoparticles can be prepared with structured morphology surface and proper particle size.

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