Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 121-124.doi: 10.3969/j.issn.1673-8225.2012.01.026

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A micro-stimulation method based on fish oocytes extracts induces mouse spleen cells to express stem cell mark antigen

Ruan Guang-ping, Yao Xiang, Pang Rong-qing, Wang Jin-xiang, Ma Li-hua, Wang Qiang, Deng Yong-li, Pan Xing-hua   

  1. Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming  650032, Yunnan Province, China
  • Received:2011-05-21 Revised:2011-07-04 Online:2012-01-01 Published:2012-01-01
  • Contact: Pan Xing-hua, Chief physician, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China xinghuapan@yahoo.com.cn
  • About author:Ruan Guang-ping☆, Doctor, Attending physician, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China ruangp@126.com
  • Supported by:

    the Social Developmental Program of Yunnan Province*

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Abstract:

BACKGROUND: The mouse and human somatic cells can be induced by some special factors to a state of embryonic cells called as induced pluripotent stem cells.
OBJECTIVE: To further enhance the efficiency of fish oocytes extracts on inducing somatic cells to differentiate into pluripotent stem cells.
METHODS: We compared a new method (micro-stimulation, 0, 0.05, 0.1, 0.2, 0.4 g/L fish oocytes extracts) to improve the induction efficiency, and the traditional method (0, 0.1, 0.2, 0.4, 0.8, 1.2 g/L fish oocytes extracts) in cultured cells by adding fish oocytes extracts.
RESULTS AND CONCLUSION: Compared with the traditional method, the new micro-stimulation method could induce spleen cells to express more stem cell marker antigens OCT-3/4, Nanog, SSEA-1. It is proved that the micro-stimulation method is a more efficient way to generate pluripotent stem cells from somatic cells.

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