Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 116-120.doi: 10.3969/j.issn.1673-8225.2012.01.025

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Expression of N-methyl-D-aspartic acid receptor 2B in the subventricular zone of juvenile rats and its cell types 

Zhang Wei1, Gao Jun-ying2, Xu Jing1, Cui Gui-yun3   

  1. 1Xuzhou Medical University, Xuzhou  221002, Jiangsu Province, China; 2Department of Anatomy, Nanjing Medical University, Nanjing  210029, Jiangsu Province, China; 3Department of Neurology, Affiliated Hospital of Xuzhou Medical University, Xuzhou  221002, Jiangsu Province, China
  • Received:2011-10-10 Revised:2011-11-11 Online:2012-01-01 Published:2012-01-01
  • Contact: Cui Gui-yun, Associate professor, Master’s supervisor, Chief physician, Department of Neurology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China cuiguiyun-js@163.com
  • About author:Zhang Wei★, Studying for master’s degree, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, ChinaWzhang8310@gmail.com Gao Jun-ying★, Master, Lecturer, Department of Anatomy, Nanjing Medical University, Nanjing 210029, Jiangsu Province, ChinaJunying_gao@126.com
  • Supported by:

     the Natural Science Foundation of Jiangsu Province, No.BK2009087*; Natural Science Foundation for Colleges and Universities in Jiangsu Province, No.07KJB310119

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Abstract:

BACKGROUND: N-methy-D-aspartic acid receptor (NR) 2B can express on neural stem cells. In-depth study of its expression in subventricular zone (SVZ) of juvenile rats can help to reveal the mechanisms of neural stem cell proliferation, differentiation, migration.
OBJECTIVE: To detect the expression of NR2B in SVZ of juvenile rats and cellular localization of NR2B on the peak of expression.
METHODS: The 7, 14, 21, 28 days postnatal SD rats were used to made 5 μm frozen sections of brain tissue. The expression of NR2B in SVZ and the cellular localization of NR2B at 14 days after birth were observed.
RESULTS AND CONCLUSION: Immunohistochemistry showed that there were differential expressions of NR2B in SVZ of 7, 14, 21, 28 days postnatal rats and the expression peak of NR2B was on 14 day; The expression of NR2B in the lateral angle of lateral ventricle was higher than that of other parts; NR2B was co-localized with glial fibrillary acidic protein (GFAP), Nestin, β-Ⅲ tubulin, and ependymal cell was most obvious. However, there was no co-localization with NeuN in SVZ. The expression level of NR2B was changed following the different postnatal time. The results of double immunofluore staining implied that NR2B might play an important role in neurogenesis.

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