Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 43-46.doi: 10.3969/j.issn.1673-8225.2012.01.009

Previous Articles     Next Articles

Guinea pig adipose mesenchymal stem cells differentiate into neuron-like cells

Guo Wei1, Ji Bin1, Ma Yan2, Bi Xiao-juan2, Liu Li-zhong1   

  1. 1Department of Otolaryngology, 2Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-07-30 Revised:2011-11-08 Online:2012-01-01 Published:2012-01-01
  • Contact: Liu Li-zhong, Doctor, Chief physician, Department of Otolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China lulizhongno. 1@ 163.com
  • About author:Liu Li-zhong, Doctor, Chief physician, Department of Otolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China lulizhongno. 1@ 163.com
  • Supported by:

    Graduate Innovation Fund of Xinjiang Medical University, No. MCR010-6*

PDF

518

Abstract:

BACKGROUND: At present, most of the animal experiments about ear stem cells research are use the stem cells of the other animal species for the guinea pig model. The stem cells from guinea pig was rarely reported.
OBJECTIVE: To explore the differentiated potential of the guinea pig adipose mesenchymal stem cells into neuron-like cells.
METHODS: Guinea pigs adipose tissue were obtained, the adipose mesenchymal cells were isolated and cultured to the 3rd generation. (1) The absorbance values of cell proliferation from 1 to 10 days were tested by cck-8 kit. (2) The immunophenotype were detected by flow cytometry. (3) Differentiation of neuron-like cells was verified by nestin, glial fibrillary acidic protein (GFAP) and neurofilament protein 200 (NF200) immunofluorescence staining.
RESULTS AND CONCLUSION: (1) The growth curve of adipose mesenchymal stem cells proliferation was similar to S-shaped, the first 3 days was the incubation period after passage, the cell populations entered logarithmic growth phase from the 4th day, after the 8th day they entered into the platform period. (2) The expression rate cells immune phenotype CD29 was 86.3%, CD44 was 55.5%, CD45 was 0.4%. (3) After differentiation of nerve cells, the morphology of cells was similar to neural cells, the results of nestin, GFAP and NF200 staining were positive. Guinea pig adipose mesenchymal stem cells are easy to isolation, culture and amplification in vitro. They have the potential to differentiate into neuron-like cells

CLC Number: