Abstract:

BACKGROUND: Multiple sets of experiments confirmed that adipose-derived stem cells (ADSCs) can amplification in vitro, and can differentiated to three germinal layers under certain conditions and can be modified with gene.
OBJECTIVE: To investigate the immunogenicity and safety of mice ADSCs after vein transplantation.
METHODS: ADSCs were isolated from adipose tissue of mice; immunity markers of these cells were observed by flow cytometry. In vitro lymphocyte hyperplasy experiment was used to determine the effect of ADSCs on activated ability of lymphocyte. ADSCs were transplanted into mice model through caudal vein and observed the stimulatory function of ADSCs on T and B lymphocyte in vivo. Anatomy the mice model after transplantation and detect the tumor of the transplanted ADSCs.
RESULTS AND CONCLUSION: ADSCs could culture in vitro and express the immunity markers of mesenchymal stem cells. When co-culture with lymphocyte, ADSCs could not stimulate the proliferation of lymphocyte efficiency. After transplantation through caudal vein, ADSCs could not stimulate immunological reaction of lymphocyte, but ADSCs could activate B lymphocyte and the humoral immunity. No tumor formation and tissue distortion were observed after transplantation. ADSCs have low immunogenicity, and could not stimulate the immune system of host cells and had a good safety.