Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 51-55.doi: 10.3969/j.issn.1673-8225.2012.01.011

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Isolation and culture of mouse adipose-derived stem cells and the expression of hematopoietic modulation factors before and after osteogenic induction

Hao Dan1, Duan Xian-lin1, Bi Xiao-juan2, Chang Xiang-ping1, Ma Yan2, Qu Jian-hua1, Yuan Hai-long1, Jiang Ming1, 2   

  1. 1Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 2Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-09-16 Revised:2011-10-24 Online:2012-01-01 Published:2012-01-01
  • Contact: Jiang Ming, Master’ s supervisor, Professor, Chief physician, Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, Chinajiangmingyy@yahoo.com.cn
  • About author:Hao Dan★, Studying for master’s degree, Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China haodan2000@163.com
  • Supported by:
    the Natural Scinence Foundation of Xinjiang Uygur Autonomous Region, No. 200821113*; Special Foundation of Stem Cells, the First Affiliated Hospital of Xinjiang Medical University, No. 2010GXB02*
     

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Abstract:

BACKGROUND: There are few reports about the isolation and culture of mouse adipose-derived stem cells in vitro and the expression of hematopoietic modulation factors before and after osteogenic induction.
OBJECTIVE: To investigate the isolation and culture of mouse adipose-derived stem cells (ADSCs) and molecule level of hematopoietic modulation factors before and after osteogenic induction in vitro.
METHODS: ADSCs were isolated from male C57BL/6 mouse by digesting adipose tissue with collagen typeⅠ, and expanded to three passages after primary culture in a control medium. Then the cells underwent the adipogenic and osteogenic induction.
RESULTS AND CONCLUSION: ADSCs grew as spindle-shaped adherent cells when cultured in vitro, and could stably proliferate and be passed. The cells’ surface antigen phenotype was analyzed by flow cytometry, and the expression of CD29 was strongly positive, CD34 was lower, the expression of CD106 and CD49d was negative. ADSCs incubated in the adipogenic medium and osteogenic medium were stained positively. The expression of hematopoietic modulation factors-catenin was the highest, the stromal cell derived factor-1 and N-cadherin was followed by, the expression of the other factors such as stem cell factor, macrophage inflammatory protein-1α, ligand jagged1 and granulocyte macrophage colony-stimulating factor were all lower. It indicates that the cells from adipose tissue are ADSCs, and the expression of hematopoietic modulation factors is found before and after osteogenic induction, but has no significant difference. 

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