Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 31-34.doi: 10.3969/j.issn.1673-8225.2012.01.006

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Differentiation of human bone marrow derived mesenchymal stem cells into epidermal-like cells induced by human amniotic membrane

Sa Ya-lian, Hua Ying-kun, Gao Jian-mei, Peng Zheng-guo, Dong Hong, Yan Xin-min   

  1. Institute of Clinical and Basic Science Pesearch, Yunnan Province First People’s Hospital (Kunhua Hospital Affiliated to Kunming Medical University), Kunming  650032, Yunnan Province, China
  • Received:2011-06-13 Revised:2011-07-28 Online:2012-01-01 Published:2012-01-01
  • About author:Sa Ya-lian☆, M.D., Ph.D., Associate chief technician, Institute of Clinical and Basic Science Research, Yunnan Province First People’s Hospital (Kunhua Hospital Affiliated to Kunming Medical University), Kunming 650032, Yunnan Province, China sa_yl@yahoo.com.cn
  • Supported by:

     the Natural Science Foundation of Yunnan Province, No. 2004C0015R*, 2007C0034R*

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Abstract:

Abstract
BACKGROUND: Cytokines are mostly used for inducing the differentiation of bone marrow mesenchymal stem cells (BMSCs) into epidermal-like cells, and human amniotic membrane is less applied to induce the differentiation of BMSCs through co-culture method.
OBJECTIVE: To explore the feasibility of human BMSCs into epidermal-like cells by using human amnion membrane.
METHODS: Human BMSCs were isolated, cultured, expanded and identified by cell morphology and expression of CD29, CD44 with flow cytometre. The 5th passage BMSCs were co-cultured with human amniotic membrane for 12 days.
RESULTS AND CONCLUSION: Human BMSCs displayed a fibroblast-like morphology and the positive cells of CD29 and CD44 were 96.6% and 94.6%, respectively. After co-culture with human amnion for 12 days, the BMSCs differentiated into epidermal-like cells with diffuse Pan-CK, focal CK19. These findings indicate that human amnion can induce human BMSCs to differentiate into epidermal-like cells.

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