Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9977-9980.doi: 10.3969/j.issn.1673-8225.2011.53.024

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Different combinations and doses of hormone influence the superovulation of different mouse strains

Qiu Ze-wen1, Jiang Wu-qi2, Gao Li-bo1, Luo Guang-bin2, Chen Jun1, Zhan Hong-wei1, Gao Wen-ting1, Wang Jing-yu1   

  1. 1Experimental Animal Center, Dalian Medical University, Dalian  116044, Liaoning Province, China
    2Shengyang Agriculture University, Shengyang  110000, Liaoning Province, China
  • Received:2011-06-13 Revised:2011-09-14 Online:2011-12-31 Published:2011-12-31
  • Contact: Wang Jing-yu, Doctor, Professor, Experimental Animal Center, Dalian Medical University, Dalian 116044, Liaoning Province, China
  • About author:Qiu Ze-wen★, Master, Laboratory technician, Experimental Animal Center, Dalian Medical University, Dalian 116044, Liaoning Province, China qiuzewens@163.com

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Abstract:

BACKGROUND: Superovulation is affected by many factors, such as animal strain, nutritional level, age, stage of estrous cycle, light, method, hormone kind and dose used in superovulation. Hormone dose and animal strain are key factors among them.
OBJECTIVE: To explore the effects of different dosage combinations of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) on the superovulation outcome in different mouse strains.
METHODS: Different dosage combinations of PMSG and HCG were used to treat ICR, KM and BALB/c mice for superovulation, respectively. The total number of embryos, the average number of embryos, the number of good embryo and the rate of good embryo were compared among different mouse strains treated with hormone.
RESULTS AND CONCLUSION: Dosage combination of 5 IU PMSG+7 IU HCG was the best hormone combination for ICR and KM mice, while the best hormone combination for BALB/c mice was 3 IU PMSG+5 IU HCG. When these three mouse strains were treated by 5 IU PMSG+7 IU HCG, the average number of embryo and the rate of good embryo of ICR and KM mice were significantly higher than that of the BALB/c mice (P < 0.05). These findings demonstrate that in order to obtain more embryos for relevant experiments, ICR and KM mice and their inbred strains should be used for superovulation, especially the internationally used ICR mice.

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