Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9965-9968.doi: 10.3969/j.issn.1673-8225.2011.53.021

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Cryopreservation of the lung from a non-hear-beating donor

Dong Qing, Cui Jian, Han Jing-quan, Yan Yu-bo, Cao Shou-qiang, Zhang Kai   

  1. Department of Thoracic Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Received:2011-08-12 Revised:2011-09-16 Online:2011-12-31 Published:2011-12-31
  • Contact: Cui Jian, Chief physician, Professor, Department of Thoracic Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • About author:Dong Qing★, Master, Physician, Department of Thoracic Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China dongqing100859@163.com
  • Supported by:

    Foundation for Harbin Excellent Subject Leaders, No.  2011RFXYS077*

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Abstract:

BACKGROUND: There are two ways to cryopreserve the lung from non-heart-beating donors: one is in situ thoracic cavity internal surface cooling technique, which has been confirmed by successful transplantation in the clinic, and the other is in situ endotracheal venting cooling technique.
OBJECTIVE: To evaluate the feasibility of combining in situ thoracic cavity internal surface cooling technique with in situ endotracheal venting cooling technique in cryopreserving the lung from non-heart-beating donor.
METHODS: Wister rats were randomly divided into three groups: in situ endotracheal venting hypothermy group (venting group), cool gas was insufflated into the trachea; in situ thoracic cavity internal surface hypothermy group (surface group): cool physiological saline was persistently injected into bilateral thoracic cavity; and combination group: cool gas insufflation combined with physiological saline injection into bilateral thoracic cavities
RESULTS AND CONCLUSION: The combination group produced better effects than the surface group and venting group (P < 0.05), and the temperature of trachea and lung parenchyma could be lowered to preservation temperature within 60 minutes. These findings suggest the feasibility of in situ thoracic cavity internal surface cooling technique and in situ endotracheal venting cooling technique used in lung transplantation.

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