Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9202-9206.doi: 10.3969/j.issn.1673-8225.2011.49.019

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Regulation of Wnt signaling pathway involved in the differentiation of hair follicle stem cells induced by lithium chloride

Yang Bin1, 2, Xiang Meng-juan2   

  1. 1Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing  100144, China
    2Guangzhou Medical University, Guangzhou  510182, Guangdong Province, China
  • Received:2011-07-20 Revised:2011-10-18 Online:2011-12-03 Published:2011-12-03
  • Contact: Xiang Meng-juan, Master, Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China Rico730@126.com
  • About author:Yang Bin☆, Doctor, Professor, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China; Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China ybdoctor_psh@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30772099*; the Natrual Science Foundation of Beijing, No.7112111*

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Abstract:

BACKGROUND: Previous studies showed that hair follicle stem cells (HFSCs) can undergo committed differentiation induced by lithium chloride (LiCl), but relating molecular regulation mechanism undisclosed.
OBJECTIVE: To observe the changes of downstream molecules and target genes in Wnt signaling pathway during exogenous factor LiCl intervenes HFSCs and to investigate the role of LiCl in committed differentiation of HFSCs.
METHODS: HFSCs were isolated from incised scalps of patients treated with rhytidectomy by two-steps enzyme digestion followed surgical microscope operation. HFSCs were subjected to antibodies-fluorescein isothiocy of cytokeratin 19 for identification. HFSCs was intervened by exogenous factor LiCl.
RESULTS AND CONCLUSION: After 5 days HFSCs treated with LiCl, HFSCs volume got larger and the nucleus/cytoplasm ratio was reduced. In Wnt signaling pathway, the mRNA expression level of β-catenin and cyclinD1 increased, GSK3β and Tcf3 decreased, accompanied with c-myc increased at a lower concentration of LiCl and inversing decreased. LiCl can affect the expression level of β-catenin, GSK3β, Tcf3 ,c-myc and cyclinD1 involved in Wnt signal pathway, which may play an important role in HFSC differentiation.

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