Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9198-9201.doi: 10.3969/j.issn.1673-8225.2011.49.018

Previous Articles     Next Articles

Transplantation of human amnion membrane mesenchymal stem cells via tail vein for the treatment of cerebral ischemia –reperfusion injury in rats

Xu Ying1, Ling Wei-hua2, Lu Shi-qi1, Fang Qi3, Li Jun-gen2   

  1. 1Emergency Medicine, 2Emergency Surgery; 3Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou  215006, Jiangsu Province, China
  • Received:2011-08-11 Revised:2011-10-27 Online:2011-12-03 Published:2011-12-03
  • Contact: Lu Shi-qi, Doctor, Chief physician, Emergency Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China papersub@sina.com
  • About author:Xu Ying★, Studying for master’s degree, Attending physician, Emergency Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China

PDF

339

Abstract:

BACKGROUND: Amniotic mesenchymal stem cells are an ideal seed cells sources for regenerative medicine.
OBJECTIVE: To investigate the effect of human amnion membrane mesenchymal stem cells (hAMSCs) via tail vein for the treatment of cerebral ischemia injury.
METHODS: The rat models of middle cerebral artery infarction were established, and underwent reperfusion at 2 hours after ischemia. 1.0×105 hAMSCs were transplanted through tail vein after 24 hours cerebral ischemia and the neurological severity score (NSS) was evaluated after 2 and 4 weeks cerebral ischemia. The rat without transplantation was as control.
RESULTS AND CONCLUSION: After cerebral ischemia injury, the neurological score of rats was increased significantly; at 1 week after AMSCs transplantation, the neurological score of rats was decreased significantly (P < 0.05); at 2 and 4 weeks after AMSCs transplantation, the neurological score was further improved. Immunofluorescence staining showed that there were green fluorescence BrdU-positive cells in the rats cerebral tissue transplanted with hAMSCs, and the positive cells were mainly located around the ischemic area, subcortex and the lateral ventricle. It indicates that tail vein transplantation of hAMSCs can migrates to the ischemia area and improve the neurological function of rats.

CLC Number: