Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8325-8328.doi: 10.3969/j.issn.1673-8225.2011.44.039

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Transplantation of freeze-dried amnion after irradiation sterilization for corneal alkali burn

Teng Dan1, Luan Xin-yao1, Xu Ni-na1, Yu Li-ping1, Li Wen-ji1, Liu Yan-hong1, He Wei2   

  1. 1Shenyang Green Valley Bio-tech Industry Co.,Ltd., Shenyang  110163, Liaoning Province, China
    2He Eye Hospital, Shenyang  110034, Liaoning Province, China
  • Received:2011-06-14 Revised:2011-09-08 Online:2011-10-29 Published:2011-10-29
  • Contact: He Wei, M.D., Chief physician, Professor, He Eye Hospital, Shenyang 110034, Liaoning Province, China hewei0111@163.com
  • About author:Teng Dan★, Master, Shenyang Green Valley Bio-tech Industry Co.,Ltd., Shenyang 110163, Liaoning Province, China zhenchengsky@tom.com

Abstract:

BACKGROUND: The freeze-dried amnion after radiation sterilization is easy to keep and use. However, whether the amnion’s activity will be reduced and clinical effect will be affected after these treatments have been rarely reported.
OBJECTIVE: To evaluate the repair function of freeze-dried amnion after radiation sterilization on rabbit cornea.
METHODS: Nine healthy adult rabbits were taken and their corneas were burned with 1 mol/L NaOH. The left corneas served as test group and the right corneas as control group. The freeze-dried amnion was planted into the test group but not planted into the control group. Within 30 days after surgery, corneal clearness, neovascularization and recovery of upper cornea were daily observed through the use of slit lamp. 
RESULTS AND CONCLUSION: At 30 days after surgery, corneal clearness, neovascularization and recovery of upper cornea were significantly better in the test group than in the control group (P < 0.05). Compared with the control group, upper cornea was better recovered, matrix fibers were more likely in order, infiltration of inflammatory cells was slighter, and there were fewer newly formed vessels in the test group. These findings suggest that freeze-dried, irradiated amnion can alleviate inflammatory response, promote recovery of upper cornea and reduce neo-vascularization after transplantation into alkali burned rabbit cornea. 

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