Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8293-8296.doi: 10.3969/j.issn.1673-8225.2011.44.031

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Ischemic preconditioning prevents ischemia/reperfusion injury during isolation of hepatocytes

Liang Shan, Shi Yong-zhong, Nie Sheng-dan, Xu Wei, Li Zhuo-ri   

  1. People’s Hospital of Hunan Province, Changsha  410005, Hunan Province, China
  • Received:2011-04-26 Revised:2011-07-10 Online:2011-10-29 Published:2011-10-29
  • Contact: Li Zhuo-ri, Doctor, Chief physician, People’s Hospital of Hunan Province, Changsha 410005, Hunan Province, China lzr59@hotmail.com
  • About author:Liang Shan★, Master, Physician, People’s Hospital of Hunan Province, Changsha 410005, Hunan Province, China liangshan5460@126.com
  • Supported by:

    Key Program of Department of Science and Technology of Hunan Province, No. 2008sk2004*

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Abstract:

BACKGROUND: There have been few studies regarding whether ischemic preconditioning (IPC) can alleviate ischemia/reperfusion injury and improve the function of residual liver during the isolation of hepatocytes.
OBJECTIVE: To investigate the preventive effects of ischemia preconditioning on hepatocyte isolation and ischemia/reperfusion injury of residual liver.
METHODS: Twelve Sprague-Dawley rats were randomly and equally divided into partial hepatectomy group and IPC group. The obtained tissues were isolated by a modified four-step collagenase perfusion technique. At the same time, rat serum was collected before and 1 day after surgery.
RESULTS AND CONCLUDION: Hepatocyte viability, hepatocyte proliferation, albumin (ALB) synthesis and superoxide dismutase (SOD) levels were significantly greater, while lactate dehydrogenase (LDH), glutamate-pyruvate transaminase (GPT) and malondialdehyde (MDA) levels were significantly decreased, in the IPC group than in the partial hepatectomy group (P < 0.05). There were no significant differences in ALB, LDH, GPT, SOD and MDA levels between partial hepatectomy and IPC groups (P > 0.05). These results showed that IPC can reduce ischemia/reperfusion injury during hepatocyte isolation. The mechanism may be related to self-induced IPC and the ability of anti-oxidant and anti-oxygen free radicals. But IPC does not produce obvious effects on the function of residual liver of donor rats.

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