Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8285-8288.doi: 10.3969/j.issn.1673-8225.2011.44.029

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Comparison of differentiation and proliferation efficiency from CD4+ cells to Th17 cells induced by three different methods

Yin Tai-lang1, Zou Yu-jie1, Yang Jing1, He Fan2, Li Sai-jiao1, Wu Geng-xiang1, Li Xing1, Wang Ya-qin1, Luo Jin1, Li Wei1, Xu Wang-ming1   

  1. 1Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
    2Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
  • Received:2011-07-25 Revised:2011-09-13 Online:2011-10-29 Published:2011-10-29
  • Contact: Yang Jing, Doctor, Professor, Chief physician, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China Correspondence to: He Fan, Doctor, Lecturer, Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
  • About author:Yin Tai-lang☆, Studying for doctorate, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China reproductive@126.com

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Abstract:

BACKGROUND: Th17 cells participate in the occurrence of many inflammatory diseases, but their induced differentiation efficiency remains poorly clear.
OBJECTIVE: To compare the differentiation and proliferation efficiency from CD4+ cells to Th17 cells induced by three different methods.
METHODS: Three methods to induce Th17 cells in vitro are as follows: (1) stimulation with CD3 and CD28 antibodies followed by addition of interleukin (IL)-6 and transforming growth factor-β (TGF-β) in the culture medium and finally culture for 3 days; (2) based on method (1), addition of extra IL-1β and tumor necrosis factor-α (TNF-α) and culture for 3 days; (3) based on method (2), washing out previous cytokines on the 3rd day, culture for 2 days, stimulation with CD3 and CD28 antibodies again, addition of IL-23 and finally culture for 3 days.
RESULTS AND CONCLUSION: The proportion of Th17 cells in CD4+T cells was (8.5±2.8)%, (26.9±4.3)%, and (44.3±5.5)% after induced by three above-mentioned methods, respectively, with significant difference among these three methods (P < 0.01). These findings suggest that addition of IL-1β, TNF-α and IL-23 into the culture medium facilitates the induced differentiation from CD4+ cells to Th17 cells.

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