Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8281-8284.doi: 10.3969/j.issn.1673-8225.2011.44.028

Previous Articles     Next Articles

Intravenous infusion of hydrogen peroxide upregulates CD4+Foxo3+ regulatory T cells in mouse spleen and peripheral blood

Gu Dong-shu, Liu Wen, Gu Wei-wang   

  1. Laboratory Animal Center, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-08-07 Revised:2011-09-09 Online:2011-10-29 Published:2011-10-29
  • Contact: Gu Wei-wang, Doctoral supervisor, Professor, Laboratory Animal Center, Southern Medical University, Guangzhou 510515, Guangdong Province, China guww100@163.com
  • About author:Gu Dong-shu, Assistant experimentalist, Laboratory Animal Center, Southern Medical University, Guangzhou 510515, Guangdong Province, China rmjy1981@qq.com
  • Supported by:

    the Science and Technology Development Program of Guangdong Province, No. 2009B060300008*

Abstract:

BACKGROUND: The naturally formed regulator T cells have been confirmed exert important function and role in maintaining organism’s homeostasis, inhibiting inflammation, prevent against transplantation rejection, inhibiting anti-tumor immune response, and preventing and treating auto-immune diseases.
OBJECTIVE: To investigate the effects of intravenous infusion of hydrogen peroxide (H2O2) on CD4+CD25+Foxo3+ regulatory T cells in the BALB/c mice.
METHODS: Twelve male BALB/c mice of specific pathogen free level, 6 weeks old, were randomly divided into two groups, H2O2 treatment group and PBS group. The mice in the H2O2 treatment group were injected 250 μL PBS with 50 mmol/L H2O2 via tail vein. The mice in the PBS group were injected 250 μL PBS via tail vein. At 2 weeks post injection, all mice were killed to collect peripheral blood, spleen and thymus. Flow cytometry was used to detect the proportion of CD4+CD25+Foxp3 T cells.
RESULTS AND CONCLUSION: The proportion of CD4+Foxp3+ T cells and CD4+Foxp3+/CD4+ cells in the peripheral blood and spleen of mice in the H2O2 treatment group was significantly higher than that in the PBS group (P < 0.05). These findings suggest that intravenous injection of H2O2 can increase the proportion of CD4+Foxp3+ regulatory T cells in the mouse peripheral blood and spleen.

CLC Number: