Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8272-8276.doi: 10.3969/j.issn.1673-8225.2011.44.026

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Nuclear factor-kappa B activity in peripheral blood mononuclear cells from patients undergoing maintenance hemodialysis and its clinical significance

Wang Jing-fu1, Zhang Kun1, Hu Gui-cai1, Xu Su-xin2, Guo Feng-ling1, Zhao Ya-juan1, Duan Shu-zhong1, Zhu Ju-ping1, Xing En-hong3   

  1. 1Department of Nephrology, 3Central Laboratory, Affiliated Hospital of Chengde Medical College, Chengde  067000, Hebei Province, China; 2Department of Laboratory Medicine, Luanping County Center for Disease Control and Prevention, Luanping  068250, Hebei Province, China
  • Received:2011-06-28 Revised:2011-07-27 Online:2011-10-29 Published:2011-10-29
  • About author:Wang Jing-fu★, Master, Associate chief physician, Department of Nephrology, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China wangjingfu1968@ sina.com
  • Supported by:

    Science and Technology Research and Development Plan Program of Chengde City, No. 201021051*

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Abstract:

BACKGROUND: The nuclear factor-κB (NF-κB) activity in peripheral blood mononuclear cells (PBMCs) from patients undergoing maintenance hemodialysis (MHD) before renal transplantation has been found to be increased abnormally.
OBJECTIVE: To investigate NF-κB activity in PBMCs from patients underlying MHD before renal transplantation and its correlation with high sensitive C-reactive protein (hs-CRP) levels and malnutrition.
METHODS: The nutritional status, anthropometric indicators, blood biochemistry, the NF-κB p65 activity in PBMCs and serum hs-CRP level in 71 patients undergoing MHD before renal transplantation and healthy controls were detected. MHD patients were divided into non-inflammatory group (< 3 mg/L) and slight inflammation group (≥ 3 mg/L) according to the hs-CRP levels.
RESULTS AND CONCLUSION: NF-κB activity in PBMCs and serum hs-CRP levels were significantly higher in MHD patients than in healthy controls (P < 0.001). The number of malnutrition patients, the score of modified quantitative subjective global assessment of nutrition (MQSGA), and the NF-κB activity in PBMCs as well as serum hs-CRP level were significantly higher in the slightly inflammatory group than in the non-inflammatory group (P ≤ 0.001 or P < 0.01 or P < 0.05). The hemoglobin, serum albumin, serum prealbumin, mid-arm muscle circumference, mid-arm circumference and grasp force were all significantly lower in the non-inflammatory group than in the non-inflammatory group (P ≤0.001) and the NF-κB activity in PBMCs were significantly higher in the non-inflammatory group than in the healthy control group (P ≤ 0.001), there was no significant difference in serum hs-CRP levels between non-inflammatory group and healthy control group (P > 0.05). Results showed that NF-κB was abnormally activated in PBMCs in patients underlying MHD before renal transplantation; with worsened inflammatory reaction, serum hs-CRP levels and the abnormal activation of NF-κB would be increased and the degree of malnutrition would be more serious. These finding demonstrates that the slight inflammatory status could be detected and malnutrition could be predicted earlier according to NF-κB activity in PBMCs than serum hs-CRP levels.

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