Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8243-8246.doi: 10.3969/j.issn.1673-8225.2011.44.019

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Protective effects of non-wounded leg ischemic postconditioning on ischemic reperfusion injury of donor pancreas graft in rats

Zhang Zhao-hui1, Wang Wei-zhong2, Li Xi1, Niu Wan-cheng1, Chen Dang-ying1, Zhang Yun-min1   

  1. 1Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou  221004, Jiangsu Province, China
    2Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shanaxi Province, China
  • Received:2011-04-12 Revised:2011-08-06 Online:2011-10-29 Published:2011-10-29
  • Contact: Li Xi, Master, Chief physician, Master’s supervisor, Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China Lixi97@163.com
  • About author:Zhang Zhao-hui☆, Doctor, Associate chief physician, Master’s supervisor, Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China zzhlyn@163.com

Abstract:

BACKGROUND: Ischemic preconditioning and ischemic postconditioning are effective methods to alleviate ischemia/reperfusion injury.
OBJECTIVE: To investigate the effects of non-wounded leg ischemic postconditioning on ischemic reperfusion injury of donor pancreas graft in rats and to analyze the possible mechanism.
METHODS: The control group consisted of six normal SD rats suffering from sham operation and 12 streptomycin-induced diabetic SD rats were randomly and evenly assigned to two groups: In the ischemia/reperfusion group, rats received pancreas transplantation normally, without additional intervention. In the ischemic postconditioning group, rats underwent non-wounded leg ischemic postconditioning before transplantation. The blood glucose, malondialdehyde (MDA) and superoxide dismutase (SOD) in graft tissue were monitored, and apoptotic cells were stained by TUNEL technique at 2 hours after long-time reperfusion. RESULTS AND CONCLUSION: The blood glucose and MDA in the ischemia/reperfusion group were significantly higher compared with the ischemic postconditioning group after reperfusion (P < 0.01). SOD level in the ischemia/reperfusion group was significantly lower than that in the ischemic postconditioning group (P < 0.01). The apoptotic index in the ischemia/reperfusion group was significantly higher than that in the ischemic postconditioning group (P < 0.01). Results showed that non-wounded leg ischemic postconditioning can protect rat pancreas graft from ischemic reperfusion injury during pancreas transplantation. The possible mechanisms may be related to releasing oxygen radical and reducing cell apoptosis after reperfusion.

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