Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8237-8242.doi: 10.3969/j.issn.1673-8225.2011.44.018

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Embryonic mouse pancreas transplantation for treatment of diabetes mellitus

Shan Ai-jing1, Yang Jun2, Chen Xi2, Ning Guang3, Wang Zheng-ming1   

  1. 1Stem Cells Transplantation Research Center, 2Department of Surgery, 3Department of Endocrinology, Shanghai Institute of Endocrinology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai   200025, China
  • Received:2011-01-24 Revised:2011-04-20 Online:2011-10-29 Published:2011-10-29
  • Contact: Wang Zheng-ming, Ph. D., Stem Cells Transplantation Research Center, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200025, China dzmwang@ yahoo.com
  • Supported by:

    following grants to Wand Zheng Ming: the National Natural Science Foundation of China, No. 30570949*; the Major Program of Stem Cells of Shanghai Committee of Science and Technology, No. 06dj14001*; 973 Program of China, No. 2007CB947901*

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Abstract:

BACKGROUND: Embryonic pancreatic tissue is characterized by its abundance, potent in proliferation & differentiation, and minimal immunological rejection. It is widely considered as potential pancreatic endocrinological stem cells resource for treating diabetes mellitus.
OBJECTIVE: To investigate the embryonic mouse pancreatic tissue isolation technique and observe the recipients’ blood glucose regulatory effects of the grafted embryonic pancreas in an experimental diabetes mellitus mouse model.
METHODS: Pancreatic tissue from C57BL/6 mouse embryos at embryonic days 11.5-16.5 was isolated under the stereomicroscope. C57BL/6 mouse models of streptozocin-induced diabetes mellitus were established and then randomly divided into two groups: transplantation group, in which, five pieces of pancreatic tissue of mice at embryonic 16.5 days were transplanted into mouse renal capsule, and sham-operated control group, in which, 0.05 mL RPMI1640 culture medium was injected into mouse renal capsule. When blood glucose level of the transplantation group mouse was ≤ 11.2 mmol/L, the endocrine function of embryonic pancreatic tissue transplanted was detected by IPGTT and IPITT methods and then the transplanted graft was removed for observing the blood glucose relapse.
RESULTS AND CONCLUSION: Nearly intact pancreatic tissue of mice at embryonic days 11.5-16.5 could be isolated through the use of stereomicroscope. Pancreatic tissue morphology and color of mice ≤ embryonic 12.5 days were difficultly distinguished from adjacent tissue and they could only be isolated carefully according to the relationship with adjacent organs. Pancreatic tissue of mice > embryonic 12.5 days exhibited initial endocrinological tissue morphology mimic white cauliflower. Histological and ELISA examinations showed that embryonic pancreatic tissue could express and secrete insulin and the insulin level was gradually increased with developmental time. Embryonic pancreatic tissue could grow beneath the recipient renal capsule. The insulin and glucagon expression in the post-transplantational pancreatic tissue graft was increased compared with prior to transplantation. These results suggest that pancreatic tissue is a potential stem cell resource for treating the diabetes mellitus.

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