Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7537-7540.doi: 10.3969/j.issn.1673-8225.2011.40.029

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Relationship of CD44+ tumor cells and S-phase marker proliferating cell nuclear antigen in colorectal carcinoma

Wang Fu-rong1, 2, Liu Bin1, 2, 3, Su Qin-jun1, Yang Yan-li1, Qian Zhen1, Shi Min1   

  1. 1Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou   730050, Gansu Province, China
    2Institute of Pathology, Lanzhou University, Lanzhou  730000, Gansu Province, China
    3Key Laboratory of Stem Cells and Gene Medicine, Lanzhou  730000, Gansu Province, China
  • Received:2011-04-05 Revised:2011-06-11 Online:2011-10-01 Published:2011-10-01
  • Contact: Liu Bin, Chief physician, Professor, Master’s supervisor, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Institute of Pathology, Lanzhou University, Lanzhou 730000, Gansu Province, China; Key Laboratory of Stem Cells and Gene Medicine, Lanzhou 730000, Gansu Province, China liumb@189.cn
  • About author:Wang Fu-rong★, Studying for master’s degree, Attending physician, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Institute of Pathology, Lanzhou University, Lanzhou 730000, Gansu Province, China 943226263@qq.com
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 096RJZA096*

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Abstract:

BACKGROUND: An ideal surface marker of cancer stem cells has not been found in colorectal carcinoma and it is very useful to find cancer stem cells in G0/G1 phase accurately by detecting the cell cycle. 
OBJECTIVE: To investigate the expression of CD44 and proliferating cell nuclear antigen (PCNA) and the relationship between CD44+ tumor cells and S-phase marker PCNA in colorectal carcinoma.
METHODS: The expression of CD44 and PCNA were detected by tissue chip, immunohistochemical staining and double immunohistochemical staining methods in 61 cases of colorectal carcinoma and 10 cases of normal mucosa.
RESULTS AND CONCLUSION: In colorectal carcinoma, the number of PCNA+ tumor cells was remarkably more than that of CD44+ cells. There were two kinds of morphous of CD44+ tumor cells: one presented with bigger vacuolus nucleus, chromatin margination, clear nuclear membrane and nucleolus, and most of these tumor cells expressed PCNA; Another showed smaller nucleus, chromatin dense, and partly expressed PCNA. In most cases, more than 90% of CD44+ tumor cells were PCNA+, but just (3.38±2.08)% showed PCNA-. In colorectal carcinoma, most of CD44+ tumor cells expressed PCNA and stayed in S-phase. Just few CD44+/PCNA- staying in G0/G1 phase may be cancer stem cells.

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