Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7497-7500.doi: 10.3969/j.issn.1673-8225.2011.40.020

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Leukemia inhibitory factor decreases the apoptosis of neurons and promotes the proliferation of endogenous neural stem cells

Li Ya-dan1, Lin Yu-feng1, Zang Da-wei2   

  1. 1 First Center Clinical College of Tianjin Medical University, Tianjin  300192, China
    2Department of Neurology, Tianjin First Center Hospital, Tianjin  300192, China
  • Received:2011-05-08 Revised:2011-08-29 Online:2011-10-01 Published:2011-10-01
  • Contact: Zang Da-wei, M.D., Ph.D., Professor, Chief physician, Master’s supervisor, Department of Neurology, Tianjin First Center Hospital, Tianjin 300192, China zangdawei@gmail.com
  • About author:Li Ya-dan★, Studying for master’s degree, First Center Clinical College of Tianjin Medical University, Tianjin 300192, China
  • Supported by:

    the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, No. [2007]110*; the grant from Tianjin Science Technical Committee, No. 07JCYBJC09600*

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Abstract:

BACKGROUND: Studies have shown that leukemia inhibitory factor (LIF) can enhance the proliferation of endogenous neural stem cells (ENSCs), and change their differentiation direction, but no studies have domenstrated whether LIF can affect neuronal apoptosis.
OBJECTIVE: To detect the neuron apoptosis and the proliferation of ENSCs in mouse models after LIF administration. 
METHODS: Thirty-two c57BL mice were randomized into sham-operation group, LIF group, Parkinson’s disease (PD) group and control group. Except the control group, mice in the other groups were injected with 6-hydroxydopamine to make PD models. LIF group received ALZET osmium pump duct implantation, and infused with normal saline containing LIF (25 μg/kg) into the cerebrospinal fluid. PD group received ALZET osmium pump duct implantation, and only infused with normal saline into the cerebrospinal fluid. Sham-operation group received ALZET osmium pump duct implantation without the pump duct.
RESULTS AND CONCLUSION: LIF group showed a statistically significant improvement in motor functioin, compared with PD group; the number of ENSCs increased and the number of apoptosis neurons decreased significantly in the LIF group compared with PD group. It is possible that LIF is a neurotrophic factor for effectively rebuilding the degenerated central nervous system.

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