Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7489-7492.doi: 10.3969/j.issn.1673-8225.2011.40.018

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Poly-L-lysine bio-effects on the three-dimensional culture of rat adipose-derived stem cells

Lu Tan, Li Xiao-wei, Zhang Jiang-feng, Xu Hai-bin   

  1. Department of Orthopedic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang  453100, Henan Province, China
  • Received:2011-07-04 Revised:2011-07-28 Online:2011-10-01 Published:2011-10-01
  • About author:Lu Tan★, Master, Lecturer, Department of Orthopedic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China lutan1982@163.com
  • Supported by:

    Henan Provincial Science and Technology Research & Development Program in 2008, No. 082102310083*; the University Technological Innovation Program of Henan Education Bureau in 2010, No. 2010HASTIT036*

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Abstract:

BACKGROUND: Poly-L-lysine (PLL) can increase the adhesion, growth and proliferation of chondrocytes and epidermal cells.
OBJECTIVE: To investigate the biological effects of PLL on three -dimensional cultured rat adipose-derived stem cells (ADSCs).
METHODS: The molecular markers (CD29, CD34, CD44 and CD45) of ADSCs were detected by flow cytometry, and then ADSCs were cultured on coralline hydroxyapatite (CHA) coated with PLL or not, respectively.
RESULTS AND CONCLUSION: The CD29 and CD44 antigens could be detected, but the CD34 and CD45 were negative. The number of cells of PLL group was more than that of blank group, the PLL group’s determination value of MTT was increased significantly which was compared with those of the blank group (P < 0.05) and the activity of alkaline phosphatase was not increased significantly (P > 0.05). The PLL can apparently increase the capacity of ADSCs adhesion, growth and proliferation, and cannot influence the osteogenic differentiation of ADSCs.

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