Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7485-7488.doi: 10.3969/j.issn.1673-8225.2011.40.017

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Promotional effects of autogeneic platelet-rich plasma on in vitro and in vivo osteoblastic ability of adipose-derived mesenchymal stem cells

Yang Min, Wang Jian, Wang Qiang   

  1. Department of Orthopedics, Yijishan Hospital, Wannan Medical College, Wuhu  241001, Anhui Province, China
  • Received:2011-04-07 Revised:2011-06-21 Online:2011-10-01 Published:2011-10-01
  • About author:Yang Min☆, Doctor, Associate chief physician, Associate professor, Department of Orthopedics, Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China yjsyygk@sohu.com
  • Supported by:

    The National Nature Science Foundation of China, No. 81171732*; the Natural Science Foundation of Anhui Province, No. 070413091*; Medical Research Project of Anhui Province, No. 09C234*; the Natural Science Research Program of Anhui Education Bureau, No. KJ2007B041*

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Abstract:

BACKGROUND: Platelet-rich plasma (PRP) can promote in vitro rapid transformation of osteoblastic phenotype.
OBJECTIVE: To investigate the effects of PRP on in vitro and in vivo osteoblastic ability of adipose-derived mesenchymal stem cells (ADSCs).
METHODS: ADSCs form rabbits in passage 3 were cultured by osteogenic induction culture medium (control group) or osteogenic induction culture medium containing 10 mL/L PRP. ADSCs of passage 3 were implanted into hydroxyapatite/tricalcium phosphate (HA/TCP) scaffold and cultured by osteogenic induction culture medium with or without
10 mL/L PRP to detect the bone formation.
RESULTS AND CONCLUSION: Alkaline phosphatase activity increased to the maximal level on day 14 in the PRP group and on day 18 in the control group, and then gradually declined, but remained a relative high level until to day 28. Histologically Von Kossa staining showed that a lot of mineralized bone nodules which stained black adhered to the wall of inside porous scaffold in two groups. A large number of calcium deposits were found. Toluidine blue dye showed that new bone formed in the pores, which was greater in the surrounding area than in the central area. The amount of in vitro calcium deposits was greater in the control group, and the amount of new bone was more in the PRP group than the control group (P < 0.05). PRP could effectively speed up the osteogenic differentiation of ADSCs in vitro and induce bone formation in vivo.

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