Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7480-7484.doi: 10.3969/j.issn.1673-8225.2011.40.016

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In vivo construction of vascularized tissue engineering adipose via adipose-derived stem cells and exogenous vascular endothelial growth factor carrier complex in vivo

Li Hong-mian1, 2, Gao Jian-hua3, Wu Tao4, Zhao Pei-ran4, Liang Shuang-wu4   

  1. 1Postdoctoral Mobile Work Station, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan  528403, Guangdong Province, China
    2Postdoctoral Mobile Research Station, Southern Medical University, Guangzhou  510515, Guangdong Province, China
    3Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    4Research Center of Tissue Engineering, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-04-01 Revised:2011-05-15 Online:2011-10-01 Published:2011-10-01
  • Contact: Gao Jian-hua, Chief physician, Professor, Doctoral supervisor, Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China gaopsnf@yahoo.com
  • About author:Li Hong-mian☆, Doctor, Associate chief physician, Master’s supervisor, Postdoctoral Mobile Work Station, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan 528403, Guangdong Province, China; Postdoctoral Mobile Research Station, Southern Medical University, Guangzhou 510515, Guangdong Province, China binrong2112@163.com
  • Supported by:

    China Postdoctoral Foundation, No. 20090450910*; the Medical Science Pesearch Foundation of Guangdong Province, No.A2011739*; the Science and Technology Plan Program of Zhongshan City, No.20113A008*

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Abstract:

BACKGROUND: Revascularization mechanism is the decisive factor for the successful construction of tissue engineering adipose tissue.
OBJECTIVE: To observe the feasibility of construction of vasuclarized tissue engineering adipose via adipose-derived stem cells and exogenous vascular endothelial growth factor (VEGF) carrier complex in vivo.
METHODS: Adipose derived stem cells (ADSCs) were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of ADSCs were conducted. After being induced towards adipocytes for 2 weeks, 5× 1010/L passage 3 cell suspension labeled by BrdU was prepared. The experiment was divided into two groups: group A (experimental group) complex in which 0.5 mL cell suspension, 100 μL VEGF and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice; group B (control group) complex in which 0.5 mL cell suspension, 100 μL DMEM and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice.
RESULTS AND CONCLUSION: Eight weeks after operation, neogenetic vessels grew into the scaffolds and mild fiber encapsulation was observed in group A, while few vessels grew into the scaffolds and also mild fiber encapsulation was observed in group B. The wet weight of cambium in group A was higher than that in group B (P < 0.01). Hematoxylin-eosin staining showed the formation of neogenetic adipose tissues and the growth of micrangium in the implant. The number of micro vessels in group A was greater than that in group B (P < 0.01). The immunofluorescence staining of cambium showed that the cell nucleus of regenerated adipocytes and partial capillary endothel ium in groups A and B presented green fluorescence. It is feasible to prepare vasuclarized tissue engineering adipose via ADSCs and exogenous VEGF carrier complex in vivo. ADSCs and exogenous VEGF participate in neovascularization of neogenetic adipose tissue.

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