Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7463-7466.doi: 10.3969/j.issn.1673-8225.2011.40.013

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Immunoregulatory effect of human umbilical cord blood mesenchymal stem cells on proliferation of T lymphocytes through paracrine mechanism

Xu Li-nan1, Yao Zhi-cheng2, Lin Nan2, Hu Kun-peng2, Zhong Yue-si2   

  1. 1Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
    2Department of Hepatobiliary Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-07-27 Revised:2011-09-07 Online:2011-10-01 Published:2011-10-01
  • About author:Xu Li-nan☆, Doctor, Attending physician, Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China drxulinan@gmail.com
  • Supported by:

    the National Natural Science Foundation for the Youth, No. 81000674*; the Science and Technology Plan Program of Guangdong Province, No. 2010B031600313*

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Abstract:

BACKGROUND: Studies have shown that umbilical cord blood mesenchymal stem cells (UCB-MSCs) have a certain role in immune regulation, but the exact mechanism is unknown.
OBJECTIVE: To study the effect of UCB-MSCs on T lymphocytes by paracrine mechanism.
METHODS: We isolated the UCB-MSCs and T lymphocytes from the umbilical cord blood and peripheral blood. Indirect coculture system of UCB-MSCs and T lymphocytes with different proportion was constructed. The group of coculture acted as experiment group, the group of monoculture T lymphocytes as control group.
RESULTS AND CONCLUSION: UBC-MSCs presented a shuffle-type growth which liked the fibroblast in morphology; the flow cytometry showed the results of cellular surface marks: CD29(+), CD44(+), CD34(-), CD45(-), HLA-DR(-). Compared with the control group, the experiment group could dramatically inhibit the proliferation of peripheral blood T lymphocytes stimulated by phytohemagglutinin in a dose-dependent manner (P < 0.05); the concentration of interleukin-10 secreted by UCB-MSCs was significantly higher in the experiment group than the control group (P < 0.05). The ability of UBC-MSCs to inhibit the proliferation of T lymphocytes was sharply impaired by neutralization experiments. UCB-MSCs can dramatically inhibit the proliferation of peripheral blood T lymphocytes, which may depend on paracrine interleukin-10 to play an immunoregulatory role.

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