Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7455-7458.doi: 10.3969/j.issn.1673-8225.2011.40.011

Previous Articles     Next Articles

Differentiation of multipotent stem cells derived from Wharton’s Jelly into endothelial cells and myocardial cells

Tan Qiang, Ma Chun-peng, Wang Qing-sheng, Qi Xi-ming, Sun Li-min, Zou Xiao-yi, Liu Dong-tian, Bian Qiu-wu   

  1. Department of Cardiology, First Hospital of Qinhuangdao, Hebei Medical University, Qinhuangdao  066000, Hebei Province, China
  • Received:2011-04-12 Revised:2011-07-15 Online:2011-10-01 Published:2011-10-01
  • About author:Tan Qiang☆, Doctor, Associate chief physician, Department of Cardiology, First Hospital of Qinhuangdao, Hebei Medical University, Qinhuangdao 066000, Hebei Province, China qhdtanqiang@yahoo.com.cn

PDF

242

Abstract:

BACKGROUND: There are plenty of multipotent stem cells in Wharton’s Jelly that is a good stem cell resource. This kind of stem cell has the feasibility of low immunity and high proliferation.
OBJECTIVE: To investigate the feasibility of multipotent stem cells derived from Wharton’s Jelly differentiating into endothelial cells and myocardial cells.
METHODS: Multipotent stem cells were isolated from Wharton’s Jelly using the attached method.
RESULTS AND CONCLUSION: Flow cytometer results showed that multipotent stem cells were positive for CD105, CD73, CD90, CD44, CD54, and negative for CD45, CD34, CD106 and CD133. Multipotent stem cells isolated from Wharton’s Jelly could swallow ac-LDL and bind UEA-1 after addition of vascular endothelial growth factor and basic fibroblast factor. After 5-azacytidine induction, troponin Ⅰ and β-myoglobulin were positive in myocardial cells. The findings indicate that a mass of stem cells can be obtained from Wharton’s Jelly, which can differentiate into endothelial cells and myocardial cells in vitro.

CLC Number: