Abstract:

BACKGROUND: Reactive oxygen species (ROS) formation induced by treatment with different culture conditions of glucose in L6 cells is the basis for studying the pathogenesis of type 2 diabetes mellitus.
OBJECTIVE: To investigate the generation of intracellular ROS induced by treatment with different concentrations of glucose and different culture time periods.
METHODS: L6 skeletal muscle cells cultured in vitro and L6 myotube cells differentiated were treated with DMEM containing 3, 5.5 and 25 mmol/L glucose and ROS formation was determined.
RESULTS AND CONCLUSION: With prolonged culture time, intracellular ROS formation increased. ROS formation was significantly accumulated in cells treated with 25 mmol/L glucose than in cells treated with 5.5 mmol/L glucose by day 4. Glucose at a concentration of 3 and 5.5 mmol/L did not produce significant effects on ROS formation. ROS formation in L6 myotubes cultured by high concentration of glucose was more obvious than that in L6 mysoblast. Results showed that glucose concentration, culture time and the differentiation degree of in vitro cultured cells are the factors influencing ROS formation and in vitro culture of cells by glucose can be used to mimic the chronic damage effect of hyperglycemia in vivo.