Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6951-6955.doi: 小胶质细胞|姜黄素|Notch1|剂量依赖|组织工程

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Inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro

Jin Yu-ling1, Luo Hai-long2, Liu Lei3, Cheng Chun-feng4, Hou Li-chun1   

  1. 1Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
    2Department of Neurology, Hongqi Hospital of  Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China
    3Department of Pathophysiology, School of Basic Medical Sciences, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
    4Department of Neurology, Second Hospital Affiliated to Jiamusi University, Jiamusi  154003, Heilongjiang Province, China
  • Received:2011-03-04 Revised:2011-04-25 Online:2011-09-10 Published:2011-09-10
  • About author:Jin Yu-ling★，Master, Associate chief physician, Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China 29757978@qq. com
  • Supported by:

    the Natural Science Foundation of Heilongjiang Province, No. D200859*  

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Abstract:

BACKGROUND: Excessively activated microglia can release a large number of neurotoxic factors which damage nerve function.
OBJECTIVE: To investigate the inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro.
METHODS: Microglias were extracted from neonatal (within 48 hours) Wistar rats and cultured. At 3 and 7 days after addition of different concentrations of curcumin (1, 5, 20 μmol/L), cell morphology, CD11b/c expression and Notch1 mRNA expression were determined.
RESULTS AND CONCLUSION: The primary cultured microglia exhibited strong refractive capacity and a spherical morphology and were excessively activated. After intervention of curcumin, the positive rate of static cells was obviously increased, and the positive rate reached a peak on day 7 of culture. RT-PCR showed that 1, 5, 20 μmol/L curcumin can obviously inhibit Notch1 mRNA expression in the microglia, and 5 μmol/L curcumin showed the most obvious inhibitory effects (P < 0.01). These results suggest that curcumin shows a dose-dependent inhibitory effect on excessive activation of microglia cultured in vitro.

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