Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6737-6740.doi: 10.3969/j.issn.1673-8225.2011.36.020

Previous Articles     Next Articles

Stem cell factor and granulocyte colony-stimulating factor affect the migration of bone marrow mesenchymal stem cells in rats with myocardial infarction

Wu Zu-chang1,2, Wu Guo-cai1, Wu Zhi-ming1, Xiong Dan1, Lü Jun-ting1, Yang Zhi-gang1   

  1. 1Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang  524001, Guangdong Province, China
    2Institute of Clinical Medicine, the First People’s Hospital of Foshan, Foshan  528000, Guangdong Province, China
  • Received:2011-03-04 Revised:2011-04-20 Online:2011-09-03 Published:2011-09-03
  • Contact: Yang Zhi-gang, M.D., Professor, Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China yangzhigangcn@yahoo.com.cn
  • About author:Wu Zu-chang★, Master, Department of Hematology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China; Institute of Clinical Medicine, the First People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China wuzuchang@163.com
  • Supported by:

    the Science and Technology Project of Guangdong Province, No. 2008B030301021*

PDF

409

Abstract:

BACKGROUND: Only 1%-5% of the transplanted mesenchymal stem cells (MSCs) home to the myocardial infarction area through peripheral venous transplantation.
OBJECTIVE: To investigate the effects of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) on MSCs homing.
METHODS: MSCs were isolated from SD rats, and purified by adhesive-screening method. MSCs at passages 3-5 were assigned to 4 groups: groups of MSCs, SCF, G-CSF and SCF+G-CSF.
RESULTS AND CONCLUSION: Under fluorescence microscope, the number of MSCs migrating to the myocardial infarction region had significant difference among MSCs group, SCF group and G-CSF group (P > 0.05), but SCF+G-CSF group had the higher number of MSCs migrating to the myocardial infarction region than other three groups (P < 0.05). Immunofluorescence histochemistry showed that some MSCs express cardiac-specific protein cTnI. Combination of SCF and G-CSF can promote MSCs homing, and induced by microenvironment in vivo, MSCs can convert into cardiomyocyte-like cells.

CLC Number: