Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2881-2884.doi: 10.3969/j.issn.1673-8225.2011.16.008

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Preparation of rabbit costal cartilage acellular matrix

Liu Meng-meng1, Zhang Jian1, Luan Bao-hua1, Sun Liang1, Li Zhong-hua2, Wang Xiao-xia3   

  1. 1Department of Burns and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan  250021, Shandong Province, China
    2Burn Unit, Fourth People's Hospital of Jinan City, Jinan  250021, Shandong Province, China
    3Jinan Nursing Vocational College, Jinan  250000, Shandong Province, China
  • Received:2010-12-23 Revised:2011-03-09 Online:2011-04-16 Published:2013-11-11
  • Contact: Luan Bao-hua, Chief physician, Department of Burns and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China luanbaohua@hotmail.com
  • About author:Liu Meng-meng★, Studying for master’s degree, Department of Burns and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China Zhang Jian, Attending physician, Department of Burns and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China Liu Meng-meng and Zhang Jian contributed equally to this paper.
  • Supported by:

     Shandong Natural Science Foundation, No. Y2002C19*

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Abstract:

BACKGROUND: Several studies have suggested that New Zealand rabbits cartilage tissue can be used as a kind of tissue engineering scaffold material and is widely used in scientific research, including articular cartilage and ear cartilage cells of the matrix of the emergence of studies comparing fully, but adopting costal cartilages as tissue engineering cartilaginous framework of research little, yet few reports.
OBJECTIVE: Based on the preparation of costal cartilage acellular matrix from New Zealand rabbits, to discuss the feasibility of natural cartilage matrix scaffold for tissue engineering scaffolds.
METHODS: By using detergent-enzyme method, cartilaginous framework was obtained, and acellular matrix was divided into four groups of 0, 24, 48 and 96 hours according to Triton X-100 second processing time. Scanning electron microscope was used for image acquisition to calculate stents porosity, pore diameter, and then the scaffolds underwent hematoxylin-eosin, toluidine blue and Ⅱ collagen type immunohistochemical staining. The compatibility of the acellular matrix scaffolds transplanted subcutaneously was observed.
RESULTS AND CONCLUSION: The acellular matrix presented with milk-white, size uniformity with intact stained structure, and there were a lot of acid mucopolysaccharide and collagen type Ⅱ. The porosity was (61.31± 8.45)%, and the pore diameter was (32.80±5.15) μm. The biocompatibility of the transplanted acellular matrix was good at 7 days after transplantation. No obvious hyperemia and purulent occurred. These findings show that there is a good substrate composition of rabbit coastal cartilage acellular matrix scaffolds with intact and even pore structure, which can be used as tissue engineering scaffold materials.

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