Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2769-2772.doi: 10.3969/j.issn.1673-8225.2011.15.026

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Brain derived neurotrophic factor promotes tube formation of cardiac microvascular endothelial cells

Liu Shao-kun1,2,3, Cao Liang1,2,3, Zhang Liang1,2,3, Shen Xiao-tao1,2,3, Zhao Bao-yin1,2,3, Qi Xu-feng1,2,3, Qin Jun-wen1,4, Cai Dong-qing1,2,3   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), the Ministry of Science and Technology & Guangdong Province Science and Technology Committee, 4Institute of Reproductive Immunology, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2010-12-23 Revised:2011-03-05 Online:2011-04-09 Published:2013-11-06
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), the Ministry of Science and Technology & Guangdong Province, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Liu Shao-kun★, Studying for master’s degree, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), the Ministry of Science and Technology & Guangdong Province Science and Technology Committee, Jinan University, Guangzhou 510632, Guangdong Province Science and Technology Committee, China sakuelee@gmail.com
  • Supported by:

    the National 863 Program of China, No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30770886*, 30570369*, 30340038*, 30973158*; the Key Program of Guangdong Natural Science Foundation, No. 04105826*; Guangdong Grant for Science and Technology Development, No. 2004B30601007*; International Collaborated Grant of Guangdong, No. 2009B050900007*; the Fundamental Research Funds for the Central Universities, No. 21609408*

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Abstract:

BACKGROUND: It has been discovered that brain derived neurotrophic factor (BDNF) can promote endothelial cell survival and induce angiogenesis. However, the cellular and molecular mechanism regarding to BDNF-induced angiogenesis is still unknown.
OBJECTIVE: To observe the effect of BDNF on tube formation of cardiac microvascular endothelial cells (CMECs) in three-dimensional (3D) in vitro tube formation assay.
METHODS: Rat CMECs were isolated and cultured until they formed a single spheroid. These spheroids were embedded in the mixture of collagen typeⅠ-carboxymethylcellulose (CMC). Then, 50 µg/L, 70 µg/L, 100 µg/L BDNF was added into the wells respectively. The number of branch and the cumulative length of all capillary-like structure were analyzed in 24 and 48 hours after BDNF treated respectively.
RESULTS AND CONCLUSION: The capillary-like structure for each CMECs spheroid was visible in 3D collagen typeⅠ-CMC containing after 24 hours culture. It was found that BDNF could promote tube formation of CMECs. The cumulative length and the branch of capillary-like structure of 100 µg/L BDNF group was significantly longer than that other groups respectively. The cumulative length of CMECs spheroids was significantly longer after 48 hours compared with at 24 hours. The results of this study suggested that BDNF could promote tube formation of CMECs in 3D in vitro tube formation assay. The promoting effect of BDNF is dosage dependence.

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