Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2764-2768.doi: 10.3969/j.issn.1673-8225.2011.15.025

Previous Articles     Next Articles

Expression of pleiotrophin and platelet derived growth factors in rat discs with increasing age

Zhang Sheng-fei, Feng Xin-min, Tao Yu-ping, Wang Hua-feng, Wen Dan   

  1. Department of Orthopedics, Subei People’s Hospital of Jiangsu Province, Yangzhou  225001, Jiangsu Province, China
  • Received:2010-11-08 Revised:2011-01-30 Online:2011-04-09 Published:2013-11-06
  • Contact: Feng Xin-min, Master, Chief physician, Department of Orthopedics, Subei People’s Hospital of Jiangsu Province, Yangzhou 225001, Jiangsu Province, China dafei8251@163.com
  • About author:Zhang Sheng-fei★, Master, Department of Orthopedics, Subei People’s Hospital of Jiangsu Province, Yangzhou 225001, Jiangsu Province, China
  • Supported by:

    the Science and Technology Foundation of Liaoning Province, No. 2006408002-4*

PDF

417

Abstract:

BACKGROUND: Pleiotrophin (PTN) directly participates in angiogenesis and plays an important role in humen degeneration of intervertebral disc under hypoxia condition. 
OBJECTIVE: To investigate the expression of PTN and platelet derived growth factor (PDGF) in rat discs at different months, so as to probe into the association of PTN and PDGF with degeneration of intervertebral disc.
METHODS: Fifty Wistar rats were divided into 5 groups according to their different months: 1-, 3-, 6-, 12 and 18-month groups. The expression of PTN and PDGF in intervertebral disc was observed by SABC immunohistochemistry. The correlation between PTN and PDGF expression was analyzed by Spearman. 
RESULTS AND CONCLUSION: The expression of PTN and PDGF in intervertebral disc decreased as rat age (1-12 months) increasing, but it increased when it came to 18-month. In the same group, the expression of PTN and PDGF was higher in cartilage end plate than in annulus fibrosus and nucleus pulposus (P < 0.05). A high correlation was presented in the expression of PTN and PDGF (P  < 0.01). The intervertebral disc of rat degenerates along with their ages of months. PTN and PDGF participate in intervertebral disc degeneration.

CLC Number: