Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2730-2734.doi: 10.3969/j.issn.1673-8225.2011.15.017

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All-trans retinoic acid induces chromatin remodeling at the promoter of mouse alkaline phosphatase

Chen Di, Sun Fen-yong   

  1. Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2010-12-31 Revised:2011-02-25 Online:2011-04-09 Published:2013-11-06
  • Contact: Sun Fen-yong, Doctor, Professor, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China sunfenyong@263.net
  • About author:Chen Di★, Studying for master’s degree, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China chendi1108@126. com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071524*

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Abstract:

BACKGROUND: Alkaline phosphatase (ALP) is an important biomarker of proliferation and differentiation in osteoblasts. All-trans retinoic acid (ATRA) up-regulates ALP expression in C3H10T1/2 cells mediated by retinoic acid receptors (RAR) and is irrelevant to the MAPK pathway.
OBJECTIVE: To reveal the mechanism of ATRA induced ALP up-regulation from chromosome structure.
METHODS: C3H10T1/2 were treated with ATRA (10-6 mol/L) for 0, 1, 6 and 12 hours, and then DNaseⅠhypersensitivity analysis was performed to identify the location of the regulatory regions of ATRA. ChIP assays were performed to analyzed transcription factors bound to regulatory regions of ATRA.
RESULTS AND CONCLUSION: DNaseⅠhypersensitivity analysis identified the location of regulatory regions of ATRA which exists in the 520 bp region of ALP promoter. ChIP assays proved that ALP up-regulated by ATRA is accompanied by a series of transcription factors binding to ALP promoter. ALP up-regulated by ATRA is accompanied by chromatin remodeling and Histone modification.

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