Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1957-1960.doi: 10.3969/j.issn.1673-8225.2011.11.014

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Influence of Wnt signaling pathway in a rat model of traumatic deep vein thrombosis

Mo Jian-wen1, Hu Hai-lan2, Bai Bo1, Li Ying1, Zhang Shu-jiang1, Chen Yi1   

  1. 1Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou  510120, Guangdong Province, China
    2Department of Orthopaedics, the Second Hospital of Guangzhou Medical College, Guangzhou  510260, Guangdong Province, China
  • Received:2010-09-30 Revised:2010-12-29 Online:2011-03-12 Published:2011-03-12
  • Contact: Bai Bo, Doctor, Professor, Chief physician, Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China drbobai@yahoo.com
  • About author:Mo Jian-wen☆, Doctor, Attending physician, Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China mjw1997@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 9151018201000035*

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Abstract:

BACKGROUND: The potential harmful of traumatic deep vein thrombosis is great. It can be complicated by pulmonary embolism or cerebral embolism. Wnt signaling pathway controls the course of many diseases, which may affect the formation of deep vein thrombosis.
OBJECTIVE: To study the role of Wnt signaling pathway in traumatic deep vein thrombosis.
METHODS: Totally 30 SD rats were divided into control and model groups. The model group was divided into 2 groups according to biological states: the crest-time of thrombosis and without thrombosis at the crest-time. Femoral vein tissues were incised at 5 days after model preparation, and total RNA was extracted. The RNA expression was determined by Genechip Rat genome 230 2.0 genechips and the changes of Wnt signaling pathway signaling pathway was analyzed. 
RESULTS AND CONCLUSION: Compared with the control group, 1 906 genes were differential expressed in the thrombosis, and 1 568 genes were differential expressed in the non-thrombosis group; compared with the non-thrombosis group, 43 genes were differential expressed in the thrombosis group, including Wnt signaling pathway of frizzled-related protein, Axam, CK2, P53, PP2A, PKA,β-catenin, c-myc, fra-1, Rac, calcium/calmodulin dependent kinase II and calcineurin were up regulated, frizzled-related protein and phospholipase C were down regulated. Wnt signaling pathway may be one of the most important pathway to control the biological states of thrombosis.

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