Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1953-1956.doi: 10.3969/j.issn.1673-8225.2011.11.013

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Changes of estrogen, nitric oxide and transforming growth factor beta 1 in rats with diabetic osteoporosis

Liu Zhong-hao, Gao Hong-wei, Xing De-guo, Zhao Xi-wu, Wang Ruo-yi, Gong Ming-zhi   

  1. First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan  250033, Shandong Province, China
  • Received:2010-09-14 Revised:2010-11-02 Online:2011-03-12 Published:2011-03-12
  • Contact: Gong Ming-zhi, Doctor, Chief physician, First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong Province, China gongmzh242@sina.com
  • About author:Liu Zhong-hao★, Master, Attending physician, First Department of Orthopaedics Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong Province, China liuxia990620@163.com
  • Supported by:

    the Plan of Science and Technology Development of Shandong Province, No. 2008GG 30002073*

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Abstract:

BACKGROUND: Diabetes mellitus and ovariectomy are two main reasons for osteoporosis, both of which can lead to variations in nitric oxide (NO) and transforming growth factor β1 (TGF-β1).
OBJECTIVE: To study the changes of estrogen, NO, and TGF-β1 in the rats with diabetic osteoporosis.
METHODS: Rats with diabetic osteoporosis were induced by streptozotocin (STZ). The bone mineral density (BMD) was measured at 4, 8, 12 and 16 weeks after model preparation; the serum estrogen levels was measured by radioimmunoassay method, serum NO levels ere detected by nitrate reduction method, serum TGF-β1 levels determined by ELISA method, and the content of TGF-β1 in the bone tissues was measured by the immunohistochemical method.
RESULTS AND CONCLUSION: With induction time prolonged, BMD of femur was gradually decreased (P < 0.05 or P < 0.01), serum NO level decreased (P < 0.05 or P < 0.01), serum TGF-β1 level increased (P < 0.01). The estrogen level in the model group was slightly decreased, but the difference had no significance (P > 0.05). Immunohistochemical results showed that TGF-β1 was mainly expressed in the osteoblast cytoplasm, and the number of positive cells was gradually decreased with induction time prolonged (P < 0.01). The changes of serum NO and TGF-β1 in bone tissues result in lower bone mineral density because of increasing bone absorption and decreasing bone formation. Nitric oxide and TGF-β1 play an important role in diabetic osteoporosis.

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