Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1949-1952.doi: 10.3969/j.issn.1673-8225.2011.11.012

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Expression of matrix metalloproteinase-3 in a rat model of subtraumatic deep vein thrombosis

Zhang Yu-bing1, Hu Ji-hong2, Li Wen3, Yao Li-qing4, Zhao Xue-ling4, Chen Miao4, Song En4, Li Yun-jian4, Wang Hui4, Zheng Yan-ke4   

  1. 1Doctoral Student of Kunming Medical University, Kunming  650032, Yunnan Province, China; 2Department of Radiology, 4Department of Orthopaedics, First Affiliated Hospital of Kunming Medical University, Kunming   650032, Yunnan Province, China; 3Department of Cardiology, Yunnan First People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2010-12-15 Revised:2011-01-19 Online:2011-03-12 Published:2011-03-12
  • Contact: Li Wen, Associate chief physician, Department of Cardiology, Yunnan First People’s Hospital, Kunming 650032, Yunnan Province, China
  • About author:Zhang Yu-bing☆, Studying for doctorate, Doctoral Student of Kunming Medical University, Kunming 650032, Yunnan Province, China zyb701017@sina. com Hu Ji-hong, Studying for doctorate, Associate professor, Department of Radiology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Zhang Yu-bing and Hu Ji-hong contributed equally to this paper.
  • Supported by:

    the Innovation Foundation for Post-graduates of Kunming Medical University, No. 2009D02*

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Abstract:

BACKGROUND: Incidence and mortality of traumatic deep vein thrombosis (TDVT) gradually increased in recent years, which generation mechanism remains unclear. Accordingly, it is necessary to explore inherent rules using gene chip technology.
OBJECTIVE: To explore the changes and rules of matrix metalloproteinases in the TDVT. 
METHODS: Ten SD rats selected from 110 served as normal controls, other 100 rats were prepared subtraumatic TDVT models by beating on bilateral posterior limbs combined with hip spica cast fixation. The femoral vein was incised at immediately, 24, 72, 120 and 168 hours after operation. Total RNA was extracted from femoral vein, and the gene expression was detected by Affymetrix 230 2.0 genechips.
RESULTS AND CONCLUSION: Expression of matrix metalloproteinase-3 was increased with prolongation of damage duration in the experimental group, suggesting matrix metalloproteinase-3 can be one of the most major factors to influence the biological states of TDVT.

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