Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1945-1948.doi: 10.3969/j.issn.1673-8225.2011.11.011

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Microglia mediates inflammation injury in mouse models of Parkinson’s disease

Lu Ming-jia1, Zhu Yi2, Sun Juan2, Yang Xue-rong2   

  1. 1Xinjiang Medical University, Urumqi  830001, Xinjiang Uygur Autonomous Region, China
    2Department of Neurology, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi  830001, Xinjiang Uygur Autonomous Region, China
  • Received:2010-10-19 Revised:2011-01-10 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhu Yi, Master, Chief physician, Doctoral supervisor, Department of Neurology, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region, China zhuyixinjiang@ hotmail.com
  • About author:Lu Ming-jia★, Studying for master’s degree, Physician, Xinjiang Medical University, Urumqi 830001, Xinjiang Uygur Autonomous Region, China circulation_0511@ 163.com
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2010211A56*

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Abstract:

BACKGROUND: Studies have demonstrated that microglia is activated in substantia nigra and striatum of Parkinson’s disease. However, the effect of released inflammatory factor on the invasion of Parkinson’s disease remains poorly understood.
OBJECTIVE: To investigate the expression of tyrosine hydroxylase (TH), integrin α and tumor necrosis factor α (TNF-α) mediated by microglia in mouse models of Parkinson’s disease.
METHODS: C57BL/6 mice were randomly divided into model group and control group. The model of Parkinson’s disease was established by peritoneal injection of paraquat to mice (10 mg/kg). Adult spontaneous motor activity was observed respectively. The level of dopamine in the substantia nigra and striatum was observed by high-performance liquid chromatography (HPLC). The expression of TH, integrin α and TNF-α in the substantia nigra pars compacta was measured by immunohistochemical staining.
RESULTS AND CONCLUSION: Compared with the control group, paraquat resulted in mouse showed a marked bypoactive behavior, such as bradykinesia, tremor, sniff and the level of dopamine in the substantia nigra and striatum was notably decreased (P < 0.05), TH declined (P < 0.05), but integrin α and TNF-α expressions increased (P < 0.05). TNF-α was positive expressed in the model group, which mainly distributed on the microglia. The inflammation injury which was mediated by microglia maybe involved in dopamine neuron damage of Parkinson’s disease.

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