Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9957-9959.doi: 10.3969/j.issn.1673-8225.2010.53.019

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Clinical significance of urinary DYZ-1 following renal transplantation

Zhou Wen-qiang 1,2, Shi Bing-yi2, Cai Ming2, Qian Ye-yong2, Li Hai-bin2, Xiao Li2, Han Yong2, Wu Hong-wen2   

  1. 1 Post-graduated Team, Academy of Military Medical Science, Beijing 100850, China; 2 Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Shi Bing-yi, Chief physician, Doctoral Supervisor, Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China shibingyi@medmail.com.cn
  • About author:Zhou Wen-qiang★, Master, Technician-in- charge, Post-graduated Team, Academy of Military Medical Science, Beijing 100850, China; Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China wenqiangzhou2050@sina.com
  • Supported by:

    the Medical Science and Technology Development Program during the Eleventh Five-Year Plan, No. 06G115*

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Abstract:

BACKGROUND: Regular monitoring of recipient urine can be used to identify function and status of renal transplant.
OBJECTIVE: To investigate the relation and clinical significance of urinary donor-derived DNA in acute rejection after kidney transplantation.
METHODS: A total of 60 female renal transplantation recipients and male donors were selected and divided into early detection group (n=40), acute rejection group (n=10), and stable function group (n=10). Urine samples were collected regularly. PCR was applied to detect DYZ-1 (special gene fragment of Y-chromosome).
RESULTS AND CONCLUSION: Donor cells were detected in urine of all the recipients on day 1 after operation. With increasing time, the intensity of donor DNA expression in urine was decreased generally. At 1 month, donor cells in urine disappeared only in 8 of 40 cases, and acute rejection happened in 1 case; in other 32 recipients, donor cells remained in urine, and 7 cases developed acute rejection; in 10 cases of acute rejection after transplantation over 3 months, 7 cases presented the expression of donor cells in urine, and 1 month following anti-rejection therapy, donor cells in urine were negative in 71.4 % cases. In 10 cases of sable renal function, only 1 case had positive expression of DYZ-1. Results show that detection of urinary DNA of donor cells could be a method for diagnosing acute renal rejection of long term recipients.

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