Abstract:

BACKGROUND: Flow-rSSO in combination with flow cytometry and PCR-SSO with high throughout subtyping are the most commonly used methods for human leucocyte antigen (HLA) genotyping at present in the countries outside China.
OBJECTIVE: To select the beads reaction patterns of different alleles in the same group and standardize the HLA genotype method, hopefully to require a more specific and rapid genotype development. 
METHODS: Genomic DNA was extracted automatically from 457 blood samples using TECAN DNA workstation. Each DNA sample was subjected to PCR amplification, molecular hybridization and Luminex magnetic beads analysis using One lambada rSSO HLA-A, B and DRB1 commercial kit. With reference to the HLA-A, B and DRBI provided by One Lambda Inc., the common alleles were analyzed and the correlation between common alleles and beads was studied.
RESULTS AND CONCLUSION: The common alleles in the same group are closely related to a certain or some different beads except the same beads reaction situation. To the organ transplantation in the clinic, the adoption of DNA genotype of middle resolution is the best choice. It not only contributes to quick screening, but also reduces the difficulty of matching. The high resolution genotype is commonly adopted in research work.