Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6887-6890.doi: 10.3969/j.issn.1673-8225.2010.37.011

Previous Articles     Next Articles

Effects of stimulus like rolling manipulation on the intracellular calcium concentration in human umbilical vein endothelial cells

Ma Hui-sheng1, Zhang Hong2, Yan Jun-tao2, Zhao Qian2, Niu Kun2　   

  1. 1 Institute of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China; 2 Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai  200437, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Zhang Hong, Doctor, Associate professor, Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China 200437 zhanghdoctor@sina.com
  • About author:Ma Hui-sheng☆, Doctor, Asssociate professor, Institute of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China madoctor@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30300462*; Innovation Program of Shanghai Municipal Education Commission, No. 09YZ138*; Key Discipline Construction Project of Shanghai for Acupuncture and Massage, No. T0302-A24*

PDF

592

Abstract:

BACKGROUND: More researches of rolling manipulation in traditional Chinese Medicine focused on the macroscopic mechanism of action on soft tissues, joints, nerves, and blood vessels. But reports about its effect on cells such as the calcium ion channels are few.
OBJECTIVE: To observe the effect of rolling manipulation on the intracellular calcium concentration of human umbilical vein endothelial cells (HUVEC).
METHODS: The normal and hypoxic HUVEC were divided into the control, massage therapy, massage + verapamil group. The control group had no interference. Cells in the massage therapy group were marked with Fluo-3/AM, and then were stimulated with 0.4 kg in pressure, 120 times per minutes in frequency, and maintained for 1 minute. The massage + verapamil group cells were marked with Fluo-3/AM, and the final concentration of 10-5 mol/L of the verapamil were added in their culture fluid. They were stimulated as the massage therapy group cells. Each group cells were observed and measured the fluorescence intensity by the confocal laser scanning microscope before and after treatment.
RESULTS AND CONCLUSION: Both stimulus like rolling manipulation and hypoxic environment could increase the intracellular calcium ion concentration, but they can be prevented by verapamil. Accordingly, it is presume that the mechanism of action of rolling manipulation is activating Ca 2+ channels, causing Ca 2+ internal flow and then increasing the intracellular Ca 2+ , which primings its therapeutic effects.

CLC Number: