Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (31): 5745-5748.doi: 10.3969/j.issn.1673-8225.2010.31.012

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Limb ischemic preconditioning in immature heart transplantation

Yang Feng, Song Jian-fei, Zheng Min, Yan Ying-wei   

  1. Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Song Jian-fei, Professor, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China
  • About author:Yang Feng★, Master, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China wineryangfeng@163.com
  • Supported by:

    the Science Foundation of Guangxi Zhuang Autonomous Region, No. 0236068*

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Abstract:

BACKGROUND: Some scholars propose that remote ischemic preconditioning can achieve ischemic preconditioning. Limb ischemic preconditioning is a self-regulating protective mechanism, whether it has protective effect on immature myocardium, and its value to heart transplantation remains unclear.

OBJECTIVE: To explore the value of limb ischemic preconditioning on the donor heart preservation by comparing immature canines limb ischemic preconditioning with the traditional method in heart preservation.
METHODS: A total of 12 canines were randomly divided into two groups, with 6 animals in each group. Femoral arteries were liberated and obstructed for 10 minites, followed by open 5 minutes, and then the chest was conventional opened after 3 cycles. The heart was dissociated, and ST.Thomas cardioplegia perfusion was infused via an inserted perfusion tube. The isolated hearts were preserved in the preservation fluid at 4 ℃ (3 mL blood was draw from the superior vena venous before the heart was removed). The treatment in the control group was similar to that of the experimental group except that the femoral arteries were not obstructed. The left ventricular myocardial samples were obtained every half an hour in the heart preservation duration     (3 hours), and the wet/dry ratio of apical myocardial was measured after preservation. The contents of malondialdehyde (MDA), superoxide enzyme (SOD), and cardiac troponin I (cTn-I) were measured. 
RESULTS AND CONCLUSION: Compared with the control group, the content of pre-cTn-I had no significant changes (P > 0.05), but the myocardial MDA and wet/dry ratio were smaller (P < 0.05), the SOD was greater (P < 0.05) after heart removal. The results demonstrated that, limb ischemic preconditioning has protective effects on immature heart. The limb ischemic preconditioning before heart removal can increase preservation outcomes of heart and decrease myocardial edema. The cTn-I content is not changed before heart removal showed that, the limb ischemic preconditioning has no damage to myocardium, thus, it is a safe protective measure.

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